Dynamic recruitment of the curvature-sensitive protein ArhGAP44 to nanoscale membrane deformations limits exploratory filopodia initiation in neurons

Milos Galic, Feng Chiao Tsai, Sean Collins, Maja Matis, Samuel Bandara, Tobias Meyer

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In the vertebrate central nervous system, exploratory filopodia transiently form on dendritic branches to sample the neuronal environment and initiate new trans-neuronal contacts. While much is known about the molecules that control filopodia extension and subsequent maturation into functional synapses, the mechanisms that regulate initiation of these dynamic, actin-rich structures have remained elusive. Here, we find that filopodia initiation is suppressed by recruitment of ArhGAP44 to actin-patches that seed filopodia. Recruitment is mediated by binding of a membrane curvature-sensing ArhGAP44 N-BAR domain to plasma membrane sections that were deformed inward by acto-myosin mediated contractile forces. A GAP domain in ArhGAP44 triggers local Rac-GTP hydrolysis, thus reducing actin polymerization required for filopodia formation. Additionally, ArhGAP44 expression increases during neuronal development, concurrent with a decrease in the rate of filopodia formation. Together, our data reveals a local auto-regulatory mechanism that limits initiation of filopodia via protein recruitment to nanoscale membrane deformations.

Original languageEnglish (US)
Pages (from-to)e03116
JournaleLife
Volume3
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

Pseudopodia
Neurons
Actins
pioglitazone
Membranes
Proteins
Neurology
Cell membranes
Myosins
Guanosine Triphosphate
Seed
Hydrolysis
Polymerization
Molecules
Synapses
Vertebrates
Seeds
Central Nervous System
Cell Membrane

Keywords

  • actin
  • cell biology
  • membrane curvature
  • neuron
  • rat

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Dynamic recruitment of the curvature-sensitive protein ArhGAP44 to nanoscale membrane deformations limits exploratory filopodia initiation in neurons. / Galic, Milos; Tsai, Feng Chiao; Collins, Sean; Matis, Maja; Bandara, Samuel; Meyer, Tobias.

In: eLife, Vol. 3, 01.01.2014, p. e03116.

Research output: Contribution to journalArticle

Galic, Milos ; Tsai, Feng Chiao ; Collins, Sean ; Matis, Maja ; Bandara, Samuel ; Meyer, Tobias. / Dynamic recruitment of the curvature-sensitive protein ArhGAP44 to nanoscale membrane deformations limits exploratory filopodia initiation in neurons. In: eLife. 2014 ; Vol. 3. pp. e03116.
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