Dynamic recompartmentalization of supported lipid bilayers using focused femtosecond laser pulses

Andreia M. Smith, Thomas R Huser, Atul N. Parikh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A direct illumination by focused femtosecond pulses from a near-infrared Ti:sapphire laser in nanojoules peak-energy regime results in a highly localized removal of phospholipids from a fluid, supported lipid bilayer submerged in aqueous media. Lipid-free gaps created in this manner exhibit long-term stability and interrupt membrane fluidity, thereby providing diffusional barriers within the membrane environment. Re-illuminating these lipid-free barriers at comparable peak energies but at higher repetition (∼200×) rapidly and selectively erases them. This in situ, reversible, maskless, multiphoton membrane photolithography provides a new means to compartmentalize and regulate membrane fluidity by erecting and erasing diffusional barriers at time scales faster than diffusional time scales. Such an ability to dynamically manipulate membrane diffusional properties should prove useful in designing synthetic models for studies of thermodynamically uphill processes including directed-molecular transport, compositional and free-energy gradients, and nonequilibrium fluctuations in biological membranes.

Original languageEnglish (US)
Pages (from-to)2422-2423
Number of pages2
JournalJournal of the American Chemical Society
Volume129
Issue number9
DOIs
StatePublished - Mar 7 2007

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Lipid bilayers
Lipid Bilayers
Ultrashort pulses
Lasers
Membranes
Membrane Fluidity
Fluidity
Lipids
Aluminum Oxide
Biological membranes
Lighting
Phospholipids
Photolithography
Sapphire
Free energy
Infrared radiation
Fluids

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Dynamic recompartmentalization of supported lipid bilayers using focused femtosecond laser pulses. / Smith, Andreia M.; Huser, Thomas R; Parikh, Atul N.

In: Journal of the American Chemical Society, Vol. 129, No. 9, 07.03.2007, p. 2422-2423.

Research output: Contribution to journalArticle

Smith, Andreia M. ; Huser, Thomas R ; Parikh, Atul N. / Dynamic recompartmentalization of supported lipid bilayers using focused femtosecond laser pulses. In: Journal of the American Chemical Society. 2007 ; Vol. 129, No. 9. pp. 2422-2423.
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