Abstract
CAMP/protein kinase (PK)A activation represents a key signaling mechanism for neurohormonal stimulation of diversified physiological processes. Using real-time, fluorescence resonance energy transfer-based imaging of PKA activity in neonatal cardiac myocytes, we report that sustained activation of PKA induced by β-adrenoceptor (βAR) dictates signaling propagation for substrate phosphorylation and myocyte contraction. Activation of βARs in wild-type myocytes induces strong and sustained PKA activities, which are rapidly attenuated on washing away agonist or adding antagonist to the cells. The sustained PKA activities promote signaling propagation to the sarcoplasmic reticulum for phosphorylation of phospholamban and increases in myocyte contraction. Addition of antagonist after βAR stimulation significantly attenuates PKA phosphorylation of phospholamban and rapidly reduces contraction rate increases. Moreover, stimulation of β1AR subtype induces PKA activities similar to those in wild-type cells. In contrast, stimulation of β2AR subtype induces strong initial activation of PKA similar to those induced by β1AR; however, the activities are rapidly decreased to baseline levels. The transient PKA activities are sufficient for phosphorylation of the overexpressed β2ARs under agonist stimulation, but not phospholamban. Further analysis reveals that phosphodiesterase 4 is the major family that shapes PKA activities under βAR stimulation. Inhibition of phosphodiesterase 4 extends β2AR-induced PKA activities, promotes PKA phosphorylation of phospholamban, and ultimately enhances myocyte contraction responses. Together, our data have revealed insights into kinetics of PKA activities in signaling propagation under neurohormonal stimulation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 770-779 |
| Number of pages | 10 |
| Journal | Circulation Research |
| Volume | 104 |
| Issue number | 6 |
| DOIs | |
| State | Published - Mar 27 2009 |
| Externally published | Yes |
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Keywords
- Adrenergic receptor
- Contraction
- Phospholamban
- Protein kinase A phosphorylation
ASJC Scopus subject areas
- Physiology
- Cardiology and Cardiovascular Medicine
Cite this
Dynamic protein kinase a activities induced by β-adrenoceptors dictate signaling propagation for substrate phosphorylation and myocyte contraction. / Soto, Dagoberto; De Arcangelis, Vania; Zhang, Jin; Xiang, Yang Kevin.
In: Circulation Research, Vol. 104, No. 6, 27.03.2009, p. 770-779.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dynamic protein kinase a activities induced by β-adrenoceptors dictate signaling propagation for substrate phosphorylation and myocyte contraction
AU - Soto, Dagoberto
AU - De Arcangelis, Vania
AU - Zhang, Jin
AU - Xiang, Yang Kevin
PY - 2009/3/27
Y1 - 2009/3/27
N2 - CAMP/protein kinase (PK)A activation represents a key signaling mechanism for neurohormonal stimulation of diversified physiological processes. Using real-time, fluorescence resonance energy transfer-based imaging of PKA activity in neonatal cardiac myocytes, we report that sustained activation of PKA induced by β-adrenoceptor (βAR) dictates signaling propagation for substrate phosphorylation and myocyte contraction. Activation of βARs in wild-type myocytes induces strong and sustained PKA activities, which are rapidly attenuated on washing away agonist or adding antagonist to the cells. The sustained PKA activities promote signaling propagation to the sarcoplasmic reticulum for phosphorylation of phospholamban and increases in myocyte contraction. Addition of antagonist after βAR stimulation significantly attenuates PKA phosphorylation of phospholamban and rapidly reduces contraction rate increases. Moreover, stimulation of β1AR subtype induces PKA activities similar to those in wild-type cells. In contrast, stimulation of β2AR subtype induces strong initial activation of PKA similar to those induced by β1AR; however, the activities are rapidly decreased to baseline levels. The transient PKA activities are sufficient for phosphorylation of the overexpressed β2ARs under agonist stimulation, but not phospholamban. Further analysis reveals that phosphodiesterase 4 is the major family that shapes PKA activities under βAR stimulation. Inhibition of phosphodiesterase 4 extends β2AR-induced PKA activities, promotes PKA phosphorylation of phospholamban, and ultimately enhances myocyte contraction responses. Together, our data have revealed insights into kinetics of PKA activities in signaling propagation under neurohormonal stimulation.
AB - CAMP/protein kinase (PK)A activation represents a key signaling mechanism for neurohormonal stimulation of diversified physiological processes. Using real-time, fluorescence resonance energy transfer-based imaging of PKA activity in neonatal cardiac myocytes, we report that sustained activation of PKA induced by β-adrenoceptor (βAR) dictates signaling propagation for substrate phosphorylation and myocyte contraction. Activation of βARs in wild-type myocytes induces strong and sustained PKA activities, which are rapidly attenuated on washing away agonist or adding antagonist to the cells. The sustained PKA activities promote signaling propagation to the sarcoplasmic reticulum for phosphorylation of phospholamban and increases in myocyte contraction. Addition of antagonist after βAR stimulation significantly attenuates PKA phosphorylation of phospholamban and rapidly reduces contraction rate increases. Moreover, stimulation of β1AR subtype induces PKA activities similar to those in wild-type cells. In contrast, stimulation of β2AR subtype induces strong initial activation of PKA similar to those induced by β1AR; however, the activities are rapidly decreased to baseline levels. The transient PKA activities are sufficient for phosphorylation of the overexpressed β2ARs under agonist stimulation, but not phospholamban. Further analysis reveals that phosphodiesterase 4 is the major family that shapes PKA activities under βAR stimulation. Inhibition of phosphodiesterase 4 extends β2AR-induced PKA activities, promotes PKA phosphorylation of phospholamban, and ultimately enhances myocyte contraction responses. Together, our data have revealed insights into kinetics of PKA activities in signaling propagation under neurohormonal stimulation.
KW - Adrenergic receptor
KW - Contraction
KW - Phospholamban
KW - Protein kinase A phosphorylation
UR - http://www.scopus.com/inward/record.url?scp=64249083698&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=64249083698&partnerID=8YFLogxK
U2 - 10.1161/CIRCRESAHA.108.187880
DO - 10.1161/CIRCRESAHA.108.187880
M3 - Article
C2 - 19213958
AN - SCOPUS:64249083698
VL - 104
SP - 770
EP - 779
JO - Circulation Research
JF - Circulation Research
SN - 0009-7330
IS - 6
ER -