Dynamic Mechanical Interactions between Neighboring Airspaces Determine Cyclic Opening and Closure in Injured Lung

Ludovic Broche, Gaetano Perchiazzi, Liisa Porra, Angela Tannoia, Mariangela Pellegrini, Savino Derosa, Alessandra Sindaco, João Batista Borges, Loïc Degrugilliers, Anders Larsson, Göran Hedenstierna, Anthony S. Wexler, Alberto Bravin, Sylvia Verbanck, Bradford J. Smith, Jason H T Bates, Sam Bayat

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objectives: Positive pressure ventilation exposes the lung to mechanical stresses that can exacerbate injury. The exact mechanism of this pathologic process remains elusive. The goal of this study was to describe recruitment/derecruitment at acinar length scales over short-time frames and test the hypothesis that mechanical interdependence between neighboring lung units determines the spatial and temporal distributions of recruitment/derecruitment, using a computational model. Design: Experimental animal study. Setting: International synchrotron radiation laboratory. Subjects: Four anesthetized rabbits, ventilated in pressure controlled mode. Interventions: The lung was consecutively imaged at ∼ 1.5-minute intervals using phase-contrast synchrotron imaging, at positive end-expiratory pressures of 12, 9, 6, 3, and 0 cm H2O before and after lavage and mechanical ventilation induced injury. The extent and spatial distribution of recruitment/derecruitment was analyzed by subtracting subsequent images. In a realistic lung structure, we implemented a mechanistic model in which each unit has individual pressures and speeds of opening and closing. Derecruited and recruited lung fractions (Fderecruited, Frecruited) were computed based on the comparison of the aerated volumes at successive time points. Measurements and Main Results: Alternative recruitment/derecruitment occurred in neighboring alveoli over short-time scales in all tested positive end-expiratory pressure levels and despite stable pressure controlled mode. The computational model reproduced this behavior only when parenchymal interdependence between neighboring acini was accounted for. Simulations closely mimicked the experimental magnitude of Fderecruited and Frecruited when mechanical interdependence was included, while its exclusion gave Frecruited values of zero at positive end-expiratory pressure greater than or equal to 3 cm H2O. Conclusions: These findings give further insight into the microscopic behavior of the injured lung and provide a means of testing protective-ventilation strategies to prevent recruitment/derecruitment and subsequent lung damage.

Original languageEnglish (US)
Pages (from-to)687-694
Number of pages8
JournalCritical Care Medicine
Volume45
Issue number4
DOIs
StatePublished - Apr 1 2017

Fingerprint

Positive-Pressure Respiration
Lung
Synchrotrons
Pressure
Mechanical Stress
Therapeutic Irrigation
Wounds and Injuries
Pathologic Processes
Artificial Respiration
Ventilation
Research Design
Radiation
Rabbits

Keywords

  • acute respiratory distress syndrome
  • assisted ventilation
  • imaging/computed tomography
  • pulmonary oedema
  • synchrotron

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Broche, L., Perchiazzi, G., Porra, L., Tannoia, A., Pellegrini, M., Derosa, S., ... Bayat, S. (2017). Dynamic Mechanical Interactions between Neighboring Airspaces Determine Cyclic Opening and Closure in Injured Lung. Critical Care Medicine, 45(4), 687-694. https://doi.org/10.1097/CCM.0000000000002234

Dynamic Mechanical Interactions between Neighboring Airspaces Determine Cyclic Opening and Closure in Injured Lung. / Broche, Ludovic; Perchiazzi, Gaetano; Porra, Liisa; Tannoia, Angela; Pellegrini, Mariangela; Derosa, Savino; Sindaco, Alessandra; Batista Borges, João; Degrugilliers, Loïc; Larsson, Anders; Hedenstierna, Göran; Wexler, Anthony S.; Bravin, Alberto; Verbanck, Sylvia; Smith, Bradford J.; Bates, Jason H T; Bayat, Sam.

In: Critical Care Medicine, Vol. 45, No. 4, 01.04.2017, p. 687-694.

Research output: Contribution to journalArticle

Broche, L, Perchiazzi, G, Porra, L, Tannoia, A, Pellegrini, M, Derosa, S, Sindaco, A, Batista Borges, J, Degrugilliers, L, Larsson, A, Hedenstierna, G, Wexler, AS, Bravin, A, Verbanck, S, Smith, BJ, Bates, JHT & Bayat, S 2017, 'Dynamic Mechanical Interactions between Neighboring Airspaces Determine Cyclic Opening and Closure in Injured Lung', Critical Care Medicine, vol. 45, no. 4, pp. 687-694. https://doi.org/10.1097/CCM.0000000000002234
Broche, Ludovic ; Perchiazzi, Gaetano ; Porra, Liisa ; Tannoia, Angela ; Pellegrini, Mariangela ; Derosa, Savino ; Sindaco, Alessandra ; Batista Borges, João ; Degrugilliers, Loïc ; Larsson, Anders ; Hedenstierna, Göran ; Wexler, Anthony S. ; Bravin, Alberto ; Verbanck, Sylvia ; Smith, Bradford J. ; Bates, Jason H T ; Bayat, Sam. / Dynamic Mechanical Interactions between Neighboring Airspaces Determine Cyclic Opening and Closure in Injured Lung. In: Critical Care Medicine. 2017 ; Vol. 45, No. 4. pp. 687-694.
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AU - Pellegrini, Mariangela

AU - Derosa, Savino

AU - Sindaco, Alessandra

AU - Batista Borges, João

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AU - Larsson, Anders

AU - Hedenstierna, Göran

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