Dynamic localization of LIN-5 and GPR-1/2 to cortical force generation domains during spindle positioning

Dae Hwi Park, Lesilee S. Rose

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

G protein signaling pathways regulate mitotic spindle positioning during cell division in many systems. In Caenorhabditis elegans embryos, Gα subunits act with the positive regulators GPR-1/2 and LIN-5 to generate cortical pulling forces for posterior spindle displacement during the first asymmetric division. GPR-1/2 are asymmetrically localized at the posterior cortex by PAR polarity cues at this time. Here we show that LIN-5 colocalizes with GPR-1/2 in one-cell embryos during spindle displacement. Significantly, we also find that LIN-5 and GPR-1/2 are localized to the opposite, anterior cortex in a polarity-dependent manner during the nuclear centration and rotation movements that orient the forming spindle onto the polarity axis. The depletion of LIN-5 or GPR-1/2 results in decreased centration and rotation rates, indicating a role in force generation at this stage. The localization of LIN-5 and GPR-1/2 is largely interdependent and requires Gα. Further, LIN-5 immunoprecipitates with Gα in vivo, and this association is GPR-1/2 dependent. These results suggest that a complex of Gα/GPR-1/2/LIN-5 is asymmetrically localized in response to polarity cues, and this may be the active signaling complex that transmits asymmetries to the force generation machinery during both nuclear rotation and spindle displacement.

Original languageEnglish (US)
Pages (from-to)42-54
Number of pages13
JournalDevelopmental Biology
Volume315
Issue number1
DOIs
StatePublished - Mar 1 2008

Keywords

  • Asymmetric division
  • G protein signaling
  • GPR-1/2
  • LET-99
  • LIN-5
  • Nuclear rotation
  • PAR-3
  • Polarity
  • Spindle positioning

ASJC Scopus subject areas

  • Developmental Biology

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