Dynamic imaging of arginine-rich heart-targeted vehicles in a mouse model

Hua Zhang, Jiro Kusunose, Azadeh Kheirolomoom, Jai W. Seo, Jinyi Qi, Katherine D. Watson, Heather A. Lindfors, Erkki Ruoslahti, Julie L. Sutcliffe, Katherine W. Ferrara

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Efficacious delivery of drugs and genes to the heart is an important goal. Here, a radiolabeled peptide-targeted liposome was engineered to bind to the heart, and the biodistribution and pharmacokinetics were determined by dynamic positron emission tomography in the FVB mouse. Efficient targeting occurred only with an exposed ligand and a dense concentration of peptide (6000 peptides/particles). Liposomes targeted with CRPPR or other arginine-rich peptides with an exposed guanidine moiety bound within 100 s after intravenous injection and remained stably bound. With CRPPR-targeted particles, the radioisotope density in the heart averaged 44 ± 9% injected dose/gram of tissue, more than 30-fold higher than in skeletal muscle. The rapid and efficient targeting of these particles can be exploited in drug and gene delivery systems and with dynamic positron emission tomography provides a model system to optimize targeting of engineered particles.

Original languageEnglish (US)
Pages (from-to)1976-1988
Number of pages13
JournalBiomaterials
Volume29
Issue number12
DOIs
StatePublished - Apr 2008

Keywords

  • Endothelium
  • Heart
  • Liposome
  • Molecular imaging
  • Peptides
  • Positron emission tomography (PET)

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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    Zhang, H., Kusunose, J., Kheirolomoom, A., Seo, J. W., Qi, J., Watson, K. D., Lindfors, H. A., Ruoslahti, E., Sutcliffe, J. L., & Ferrara, K. W. (2008). Dynamic imaging of arginine-rich heart-targeted vehicles in a mouse model. Biomaterials, 29(12), 1976-1988. https://doi.org/10.1016/j.biomaterials.2007.12.033