Dynamic imaging in living cells: windows into local signaling.

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Abstract

Highly localized changes in intracellular calcium concentration [Ca2+]i play a critical role in regulating numerous cellular functions, ranging from muscle contraction to neurotransmitter and hormone secretion to gene transcription. Fluorescent Ca2+ indicators have been invaluable tools in elucidating the role of localized changes in [Ca2+]i in regulating ion channels and other key proteins in various signaling pathways. Other techniques used to investigate localized changes in [Ca2+]i include approaches based on fluorescence resonance energy transfer, and electrophysiological measurements of ionic flux through Ca2+-sensitive channels. This Perspective discusses research using fluorescent Ca2+ indicators to study excitation-contraction coupling in cardiac myocytes, presenting both key findings and limitations of this approach. Complementary approaches useful in studying localized changes in Ca2+ and other second messengers (such as cyclic adenosine monophosphate) are also discussed.

Original languageEnglish (US)
JournalScience's STKE : signal transduction knowledge environment
Volume2003
Issue number177
StatePublished - 2003
Externally publishedYes

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Excitation Contraction Coupling
Fluorescence Resonance Energy Transfer
Second Messenger Systems
Muscle Contraction
Ion Channels
Cardiac Myocytes
Cyclic AMP
Neurotransmitter Agents
Cells
Hormones
Calcium
Imaging techniques
Transcription
Research
Genes
Muscle
Proteins
Fluxes

Cite this

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