Dynamic contrast optical coherence tomography images transit time and quantifies microvascular plasma volume and flow in the retina and choriocapillaris

Conrad W. Merkle, Conor Leahy, Vivek Srinivasan

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Despite the prevalence of optical imaging techniques to measure hemodynamics in large retinal vessels, quantitative measurements of retinal capillary and choroidal hemodynamics have traditionally been challenging. Here, a new imaging technique called dynamic contrast optical coherence tomography (DyC-OCT) is applied in the rat eye to study microvascular blood flow in individual retinal and choroidal layers in vivo. DyC-OCT is based on imaging the transit of an intravascular tracer dynamically as it passes through the field-of-view. Hemodynamic parameters can be determined through quantitative analysis of tracer kinetics. In addition to enabling depth-resolved transit time, volume, and flow measurements, the injected tracer also enhances OCT angiograms and enables clear visualization of the choriocapillaris, particularly when combined with a post-processing method for vessel enhancement. DyC-OCT complements conventional OCT angiography through quantification of tracer dynamics, similar to fluorescence angiography, but with the important added benefit of laminar resolution.

Original languageEnglish (US)
Article number#269104
Pages (from-to)4289-4312
Number of pages24
JournalBiomedical Optics Express
Volume7
Issue number10
DOIs
StatePublished - Oct 1 2016

Keywords

  • Blood or tissue constituent monitoring
  • Medical and biological imaging
  • Optical coherence tomography
  • Physiology
  • Retina scanning
  • Three-dimensional microscopy

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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