Dynamic changes in intracellular localization and isoforms of the 27-kD stress protein in human keratinocytes

R. Marla McClaren, Roslyn Rivkah Isseroff

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

We have begun to characterize the low molecular weight, 27-kD heat shock or stress protein (HSP27) in normal keratinocytes and in HaCaT, a spontaneously transformed keratinocyte line. The presence and location of HSP27 was determined by indirect immunofluorescence on fixed whole cells and immunoblot analysis of cytosolic, membrane, nuclear, and cytoskeletal cell fractions. HSP27 is localized throughout the cytoplasm of cells at 37°C. After heating at 42°C, there is a rapid (within 10 min) increase in nuclear HSP27. Two-dimensional gel analysis of whole cell HaCaT lysates identified multiple isoforms of HSP27 with different isoelectric points. The function of HSP27 is largely unknown but its presence throughout the cytoplasm of cells at 37°C, its translocation to the nucleus after cellular stress, and the presence of multiple isoforms suggest a biologic role in both stressed and unstressed human keratinocytes.

Original languageEnglish (US)
Pages (from-to)375-381
Number of pages7
JournalJournal of Investigative Dermatology
Volume102
Issue number3
StatePublished - Mar 1994

Fingerprint

Heat-Shock Proteins
Keratinocytes
Protein Isoforms
Cytoplasm
Gels
Molecular weight
Membranes
Nuclear Envelope
Isoelectric Point
Heating
Indirect Fluorescent Antibody Technique
Molecular Weight

Keywords

  • HaCaT lysates
  • heat shock proteins

ASJC Scopus subject areas

  • Dermatology

Cite this

Dynamic changes in intracellular localization and isoforms of the 27-kD stress protein in human keratinocytes. / Marla McClaren, R.; Isseroff, Roslyn Rivkah.

In: Journal of Investigative Dermatology, Vol. 102, No. 3, 03.1994, p. 375-381.

Research output: Contribution to journalArticle

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