Dynactin-dependent cortical dynein and spherical spindle shape correlate temporally with meiotic spindle rotation in Caenorhabditis elegans

Marina E. Crowder, Jonathan R. Flynn, Karen P. McNally, Daniel B. Cortes, Kari L. Price, Paul A. Kuehnert, Michelle T. Panzica, Armann Andaya, Julie A. Leary, Francis J. McNally

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Oocyte meiotic spindles orient with one pole juxtaposed to the cortex to facilitate extrusion of chromosomes into polar bodies. In Caenorhabditis elegans, these acentriolar spindles initially orient parallel to the cortex and then rotate to the perpendicular orientation. To understand the mechanism of spindle rotation, we characterized events that correlated temporally with rotation, including shortening of the spindle in the pole-to pole axis, which resulted in a nearly spherical spindle at rotation. By analyzing large spindles of polyploid C. Elegans and a related nematode species, we found that spindle rotation initiated at a defined spherical shape rather than at a defined spindle length. In addition, dynein accumulated on the cortex just before rotation, and microtubules grew from the spindle with plus ends outward during rotation. Dynactin depletion prevented accumulation of dynein on the cortex and prevented spindle rotation independently of effects on spindle shape. These results support a cortical pulling model in which spindle shape might facilitate rotation because a sphere can rotate without deforming the adjacent elastic cytoplasm. We also present evidence that activation of spindle rotation is promoted by dephosphorylation of the basic domain of p150 dynactin.

Original languageEnglish (US)
Pages (from-to)3030-3046
Number of pages17
JournalMolecular Biology of the Cell
Volume26
Issue number17
DOIs
StatePublished - Sep 1 2015

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Dyneins
Spindle Apparatus
Caenorhabditis elegans
Spindle Poles
Polar Bodies
Dynactin Complex
Polyploidy
Microtubules
Oocytes
Cytoplasm
Chromosomes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Crowder, M. E., Flynn, J. R., McNally, K. P., Cortes, D. B., Price, K. L., Kuehnert, P. A., ... McNally, F. J. (2015). Dynactin-dependent cortical dynein and spherical spindle shape correlate temporally with meiotic spindle rotation in Caenorhabditis elegans. Molecular Biology of the Cell, 26(17), 3030-3046. https://doi.org/10.1091/mbc.E15-05-0290

Dynactin-dependent cortical dynein and spherical spindle shape correlate temporally with meiotic spindle rotation in Caenorhabditis elegans. / Crowder, Marina E.; Flynn, Jonathan R.; McNally, Karen P.; Cortes, Daniel B.; Price, Kari L.; Kuehnert, Paul A.; Panzica, Michelle T.; Andaya, Armann; Leary, Julie A.; McNally, Francis J.

In: Molecular Biology of the Cell, Vol. 26, No. 17, 01.09.2015, p. 3030-3046.

Research output: Contribution to journalArticle

Crowder, ME, Flynn, JR, McNally, KP, Cortes, DB, Price, KL, Kuehnert, PA, Panzica, MT, Andaya, A, Leary, JA & McNally, FJ 2015, 'Dynactin-dependent cortical dynein and spherical spindle shape correlate temporally with meiotic spindle rotation in Caenorhabditis elegans', Molecular Biology of the Cell, vol. 26, no. 17, pp. 3030-3046. https://doi.org/10.1091/mbc.E15-05-0290
Crowder, Marina E. ; Flynn, Jonathan R. ; McNally, Karen P. ; Cortes, Daniel B. ; Price, Kari L. ; Kuehnert, Paul A. ; Panzica, Michelle T. ; Andaya, Armann ; Leary, Julie A. ; McNally, Francis J. / Dynactin-dependent cortical dynein and spherical spindle shape correlate temporally with meiotic spindle rotation in Caenorhabditis elegans. In: Molecular Biology of the Cell. 2015 ; Vol. 26, No. 17. pp. 3030-3046.
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