Duration of fasting but not diurnal variation affects the response to glucagon in healthy cats

Chen Gilor, R. Glock, S. Gilor

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The role of glucagon disturbances in diabetes is increasingly recognized. Glucagon stimulation tests (GSTs) have been described in cats previously, but information is lacking on the response of cats to glucagon under specific conditions. The aim of this study was to assess a novel protocol for GST using human-recombinant glucagon and the effect of diurnal variation and duration of fasting using this protocol in healthy cats. All intravenous doses resulted in occasional vomiting and nausea, and eventually, a 20-μg/kg intramuscular dose was chosen. Five healthy cats were then used in a repeated-measures study. Cats were free-fed regularly at 7:30 AM and 5:30 PM for 30min. In each cat, GST was performed at 7 PM after a 25-h fast (PM25), at 9 AM after a 25-h fast (AM25), and at 9 AM after a 15-h fast (AM15). Glucose and insulin concentrations were measured at -15, 0, 15, 25, 35, 45, and 60min after stimulation. Baseline and peak concentrations were compared using the Friedman test. Baseline glucose and insulin did not differ significantly between treatment groups. Peak glucose concentrations occurred at 15min and were significantly higher (P = 0.0085) at AM15 (mean ± standard deviation = 185.2 ± 43.0mg/dL) vs AM25 (144.4 ± 10.5mg/dL) and PM25 (128.0 ± 18.4mg/dL). Similarly, peak insulin concentrations occurred at 15min and were significantly higher (P = 0.04) at AM15 (1,911 ± 1,153 pg/mL) vs AM25 (739 ± 52 pg/mL) or PM25 (549 ± 366 pg/mL). In conclusion, prolonged fasting significantly blunted the glycemic response to glucagon compared with shorter fasting, but diurnal variation had no significant effect on glucose or insulin responses.

Original languageEnglish (US)
Pages (from-to)103-107
Number of pages5
JournalDomestic Animal Endocrinology
Volume53
DOIs
StatePublished - Oct 1 2015
Externally publishedYes

Fingerprint

glucagon
diurnal variation
Glucagon
fasting
Fasting
Cats
cats
duration
insulin
Insulin
Glucose
glucose
testing
nausea
vomiting
dosage
Nausea
Vomiting
diabetes

Keywords

  • Diabetes
  • Feline
  • Glucagon stimulation
  • Glucose
  • Insulin
  • Prediabetes

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Endocrinology

Cite this

Duration of fasting but not diurnal variation affects the response to glucagon in healthy cats. / Gilor, Chen; Glock, R.; Gilor, S.

In: Domestic Animal Endocrinology, Vol. 53, 01.10.2015, p. 103-107.

Research output: Contribution to journalArticle

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abstract = "The role of glucagon disturbances in diabetes is increasingly recognized. Glucagon stimulation tests (GSTs) have been described in cats previously, but information is lacking on the response of cats to glucagon under specific conditions. The aim of this study was to assess a novel protocol for GST using human-recombinant glucagon and the effect of diurnal variation and duration of fasting using this protocol in healthy cats. All intravenous doses resulted in occasional vomiting and nausea, and eventually, a 20-μg/kg intramuscular dose was chosen. Five healthy cats were then used in a repeated-measures study. Cats were free-fed regularly at 7:30 AM and 5:30 PM for 30min. In each cat, GST was performed at 7 PM after a 25-h fast (PM25), at 9 AM after a 25-h fast (AM25), and at 9 AM after a 15-h fast (AM15). Glucose and insulin concentrations were measured at -15, 0, 15, 25, 35, 45, and 60min after stimulation. Baseline and peak concentrations were compared using the Friedman test. Baseline glucose and insulin did not differ significantly between treatment groups. Peak glucose concentrations occurred at 15min and were significantly higher (P = 0.0085) at AM15 (mean ± standard deviation = 185.2 ± 43.0mg/dL) vs AM25 (144.4 ± 10.5mg/dL) and PM25 (128.0 ± 18.4mg/dL). Similarly, peak insulin concentrations occurred at 15min and were significantly higher (P = 0.04) at AM15 (1,911 ± 1,153 pg/mL) vs AM25 (739 ± 52 pg/mL) or PM25 (549 ± 366 pg/mL). In conclusion, prolonged fasting significantly blunted the glycemic response to glucagon compared with shorter fasting, but diurnal variation had no significant effect on glucose or insulin responses.",
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