Dual-specificity interaction of HIV-1 TAR RNA with tat peptide - oligonucleotide conjugates

Ching Hsuan Tung, Jihong Wang, Michael J. Leibowitz, Michael J Leibowitz

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

An oligonucleotide - peptide conjugate, having dual binding capability for a designated RNA, was designed. The peptide portion of the conjugate interacts with a folded domain in the RNA, whereas the oligonucleotide portion hybridizes with a nearby single-stranded region in the RNA. The dual specificity was proven in a model HIV-1 TAR RNA system using an RNase H cleavage assay to assess antisense binding to this RNA. The peptide portion of the conjugate was shown to confer increased specificity on the oligonucleotide.

Original languageEnglish (US)
Pages (from-to)292-295
Number of pages4
JournalBioconjugate Chemistry
Volume6
Issue number3
StatePublished - 1995
Externally publishedYes

Fingerprint

Oligonucleotides
RNA
Peptides
HIV-1
Ribonuclease H
Assays

ASJC Scopus subject areas

  • Chemistry(all)
  • Organic Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Dual-specificity interaction of HIV-1 TAR RNA with tat peptide - oligonucleotide conjugates. / Tung, Ching Hsuan; Wang, Jihong; Leibowitz, Michael J.; Leibowitz, Michael J.

In: Bioconjugate Chemistry, Vol. 6, No. 3, 1995, p. 292-295.

Research output: Contribution to journalArticle

Tung, Ching Hsuan ; Wang, Jihong ; Leibowitz, Michael J. ; Leibowitz, Michael J. / Dual-specificity interaction of HIV-1 TAR RNA with tat peptide - oligonucleotide conjugates. In: Bioconjugate Chemistry. 1995 ; Vol. 6, No. 3. pp. 292-295.
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