Dual-specificity interaction of HIV-1 TAR RNA with tat peptide - oligonucleotide conjugates

Ching Hsuan Tung, Jihong Wang, Michael J Leibowitz, Stanley Stein

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

An oligonucleotide - peptide conjugate, having dual binding capability for a designated RNA, was designed. The peptide portion of the conjugate interacts with a folded domain in the RNA, whereas the oligonucleotide portion hybridizes with a nearby single-stranded region in the RNA. The dual specificity was proven in a model HIV-1 TAR RNA system using an RNase H cleavage assay to assess antisense binding to this RNA. The peptide portion of the conjugate was shown to confer increased specificity on the oligonucleotide.

Original languageEnglish (US)
Pages (from-to)292-295
Number of pages4
JournalBioconjugate Chemistry
Volume6
Issue number3
StatePublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Organic Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

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