Dual selection of a genetic switch by a single selection marker

Yoko Nomura, Yohei Yokobayashi

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Forward engineering of synthetic genetic circuits in living cells is expected to deliver various applications in biotechnology and medicine and to provide valuable insights into the design principles of natural gene networks. However, lack of biochemical data and complexity of biological environment complicate rational design of such circuits based on quantitative simulation. Previously, we have shown that directed evolution can complement our weakness in designing genetic circuits by screening or selecting functional circuits from a large pool of nonfunctional ones. Here we describe a dual selection strategy that allows selection of both ON and OFF states of genetic circuits using tetA as a single selection marker. We also describe a successful demonstration of a genetic switch selection from a 2000-fold excess background of nonfunctional switches in three rounds of iterative selection. The dual selection system is more robust than the previously reported selection system employing three genes, with no observed false positive mutants during the simulated selections.

Original languageEnglish (US)
Pages (from-to)115-120
Number of pages6
JournalBioSystems
Volume90
Issue number1
DOIs
StatePublished - Jul 2007

Keywords

  • Directed evolution
  • Genetic circuit
  • Genetic selection
  • Genetic switch

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biotechnology
  • Drug Discovery

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