Dual polarity accurate mass calibration for electrospray ionization and matrix-assisted laser desorption/ionization mass spectrometry using maltooligosaccharides

Brian H. Clowers, Eric D. Dodds, Richard R. Seipert, Carlito B Lebrilla

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

In view of the fact that memory effects associated with instrument calibration hinder the use of many mass-to-charge (m/z) ratios and tuning standards, identification of robust, comprehensive, inexpensive, and memory-free calibration standards is of particular interest to the mass spectrometry community. Glucose and its isomers are known to have a residue mass of 162.05282 Da; therefore, both linear and branched forms of polyhexose oligosaccharides possess well-defined masses, making them ideal candidates for mass calibration. Using a wide range of maltooligosaccharides (MOSs) derived from commercially available beers, ions with m/z ratios from approximately 500 to 2500 Da or more have been observed using Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and time-of-flight mass spectrometry (TOF-MS). The MOS mixtures were further characterized using infrared multiphoton dissociation (IRMPD) and nano-liquid chromatography/mass spectrometry (nano-LC/MS). In addition to providing well-defined series of positive and negative calibrant ions using either electrospray ionization (ESI) or matrix-assisted laser desorption/ionization (MALDI), the MOSs are not encumbered by memory effects and, thus, are well-suited mass calibration and instrument tuning standards for carbohydrate analysis.

Original languageEnglish (US)
Pages (from-to)205-213
Number of pages9
JournalAnalytical Biochemistry
Volume381
Issue number2
DOIs
StatePublished - Oct 15 2008

Keywords

  • Electrospray ionization
  • Infrared multiphoton dissociation
  • Mass calibration
  • Matrix-assisted laser desorption/ionization
  • Oligosaccharide
  • Porous-graphitized carbon

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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