Dual-phone illumination-imaging system for high resolution and large field of view multi-modal microscopy

Sara Kheireddine, Ayyappasamy Sudalaiyadum Perumal, Zachary J. Smith, Dan V. Nicolau, Sebastian Wachsmann-Hogiu

Research output: Contribution to journalArticle

Abstract

In this paper we present for the first time a system comprised of two mobile phones, one for illumination and the other for microscopy, as a portable, user-friendly, and cost-effective microscopy platform for a wide range of applications. Versatile and adaptive illumination is made with a Retina display of an Apple mobile phone device. The phone screen is used to project various illumination patterns onto the specimen being imaged, each corresponding to a different illumination mode, such as bright-field, dark-field, point illumination, Rheinberg illumination, and fluorescence microscopy. The second phone (a Nokia phone) is modified to record microscopic images about the sample. This imaging platform provides a high spatial resolution of at least 2 μm, a large field-of-view of 3.6 × 2.7 mm, and a working distance of 0.6 mm. We demonstrate the performance of this platform for the visualization of microorganisms within microfluidic devices to gather qualitative and quantitative information regarding microorganism morphology, dimension, count, and velocity/trajectories in the x-y plane.

Original languageEnglish (US)
Pages (from-to)825-836
Number of pages12
JournalLab on a Chip
Volume19
Issue number5
DOIs
StatePublished - Jan 1 2019

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Lighting
Imaging systems
Microscopy
Microscopic examination
Cell Phones
Lab-On-A-Chip Devices
Mobile phones
Microorganisms
Fluorescence microscopy
Malus
Fluorescence Microscopy
Microfluidics
Retina
Visualization
Display devices
Trajectories
Imaging techniques
Costs and Cost Analysis
Equipment and Supplies
Costs

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Dual-phone illumination-imaging system for high resolution and large field of view multi-modal microscopy. / Kheireddine, Sara; Sudalaiyadum Perumal, Ayyappasamy; Smith, Zachary J.; Nicolau, Dan V.; Wachsmann-Hogiu, Sebastian.

In: Lab on a Chip, Vol. 19, No. 5, 01.01.2019, p. 825-836.

Research output: Contribution to journalArticle

Kheireddine, Sara ; Sudalaiyadum Perumal, Ayyappasamy ; Smith, Zachary J. ; Nicolau, Dan V. ; Wachsmann-Hogiu, Sebastian. / Dual-phone illumination-imaging system for high resolution and large field of view multi-modal microscopy. In: Lab on a Chip. 2019 ; Vol. 19, No. 5. pp. 825-836.
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