Abstract
Mammalian nervous system communicates mainly through chemical synapses with neurotransmitters in the presynaptic axon terminal and their receptors on the postsynaptic dendritic targets. We describe here an immuno-electron microscopic (immuno-EM) method to simultaneously label presynaptic neurotransmitters with postembedding immunogold and postsynaptic components with preembedding immunoperoxidase. This dual-immuno-EM labeling technique allows reliable distinction of the two types of EMsignals in closely related ultrastructures. The key steps of the procedure and the detailed protocol are provided.
| Original language | English (US) |
|---|---|
| Title of host publication | Neuromethods |
| Publisher | Humana Press Inc. |
| Pages | 21-33 |
| Number of pages | 13 |
| Volume | 115 |
| DOIs | |
| State | Published - 2016 |
Publication series
| Name | Neuromethods |
|---|---|
| Volume | 115 |
| ISSN (Print) | 08932336 |
| ISSN (Electronic) | 19406045 |
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Keywords
- Dual labeling
- Excitatory neurotransmitter
- Gold particles
- Immuno-EM
- Immunoperoxidase
- Postsynaptic density
ASJC Scopus subject areas
- Neuroscience(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)
- Psychiatry and Mental health
Cite this
Dual-labeling immuno-electron microscopic method for identifying pre- and postsynaptic profiles in Mammalian brain. / Liu, Xiao Bo; Cheng, Hwai Jong.
Neuromethods. Vol. 115 Humana Press Inc., 2016. p. 21-33 (Neuromethods; Vol. 115).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Dual-labeling immuno-electron microscopic method for identifying pre- and postsynaptic profiles in Mammalian brain
AU - Liu, Xiao Bo
AU - Cheng, Hwai Jong
PY - 2016
Y1 - 2016
N2 - Mammalian nervous system communicates mainly through chemical synapses with neurotransmitters in the presynaptic axon terminal and their receptors on the postsynaptic dendritic targets. We describe here an immuno-electron microscopic (immuno-EM) method to simultaneously label presynaptic neurotransmitters with postembedding immunogold and postsynaptic components with preembedding immunoperoxidase. This dual-immuno-EM labeling technique allows reliable distinction of the two types of EMsignals in closely related ultrastructures. The key steps of the procedure and the detailed protocol are provided.
AB - Mammalian nervous system communicates mainly through chemical synapses with neurotransmitters in the presynaptic axon terminal and their receptors on the postsynaptic dendritic targets. We describe here an immuno-electron microscopic (immuno-EM) method to simultaneously label presynaptic neurotransmitters with postembedding immunogold and postsynaptic components with preembedding immunoperoxidase. This dual-immuno-EM labeling technique allows reliable distinction of the two types of EMsignals in closely related ultrastructures. The key steps of the procedure and the detailed protocol are provided.
KW - Dual labeling
KW - Excitatory neurotransmitter
KW - Gold particles
KW - Immuno-EM
KW - Immunoperoxidase
KW - Postsynaptic density
UR - http://www.scopus.com/inward/record.url?scp=84978288641&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84978288641&partnerID=8YFLogxK
U2 - 10.1007/7657_2015_78
DO - 10.1007/7657_2015_78
M3 - Chapter
AN - SCOPUS:84978288641
VL - 115
T3 - Neuromethods
SP - 21
EP - 33
BT - Neuromethods
PB - Humana Press Inc.
ER -