Dual-labeling immuno-electron microscopic method for identifying pre- and postsynaptic profiles in Mammalian brain

Xiao Bo Liu, Hwai Jong Cheng

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Mammalian nervous system communicates mainly through chemical synapses with neurotransmitters in the presynaptic axon terminal and their receptors on the postsynaptic dendritic targets. We describe here an immuno-electron microscopic (immuno-EM) method to simultaneously label presynaptic neurotransmitters with postembedding immunogold and postsynaptic components with preembedding immunoperoxidase. This dual-immuno-EM labeling technique allows reliable distinction of the two types of EMsignals in closely related ultrastructures. The key steps of the procedure and the detailed protocol are provided.

Original languageEnglish (US)
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages21-33
Number of pages13
Volume115
DOIs
StatePublished - 2016

Publication series

NameNeuromethods
Volume115
ISSN (Print)08932336
ISSN (Electronic)19406045

Keywords

  • Dual labeling
  • Excitatory neurotransmitter
  • Gold particles
  • Immuno-EM
  • Immunoperoxidase
  • Postsynaptic density

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

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  • Cite this

    Liu, X. B., & Cheng, H. J. (2016). Dual-labeling immuno-electron microscopic method for identifying pre- and postsynaptic profiles in Mammalian brain. In Neuromethods (Vol. 115, pp. 21-33). (Neuromethods; Vol. 115). Humana Press Inc.. https://doi.org/10.1007/7657_2015_78