Abstract
Mammalian nervous system communicates mainly through chemical synapses with neurotransmitters in the presynaptic axon terminal and their receptors on the postsynaptic dendritic targets. We describe here an immuno-electron microscopic (immuno-EM) method to simultaneously label presynaptic neurotransmitters with postembedding immunogold and postsynaptic components with preembedding immunoperoxidase. This dual-immuno-EM labeling technique allows reliable distinction of the two types of EMsignals in closely related ultrastructures. The key steps of the procedure and the detailed protocol are provided.
| Original language | English (US) |
|---|---|
| Title of host publication | Neuromethods |
| Publisher | Humana Press Inc. |
| Pages | 21-33 |
| Number of pages | 13 |
| Volume | 115 |
| DOIs | |
| State | Published - 2016 |
Publication series
| Name | Neuromethods |
|---|---|
| Volume | 115 |
| ISSN (Print) | 08932336 |
| ISSN (Electronic) | 19406045 |
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Keywords
- Dual labeling
- Excitatory neurotransmitter
- Gold particles
- Immuno-EM
- Immunoperoxidase
- Postsynaptic density
ASJC Scopus subject areas
- Neuroscience(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)
- Psychiatry and Mental health
Cite this
Liu, X. B., & Cheng, H. J. (2016). Dual-labeling immuno-electron microscopic method for identifying pre- and postsynaptic profiles in Mammalian brain. In Neuromethods (Vol. 115, pp. 21-33). (Neuromethods; Vol. 115). Humana Press Inc.. https://doi.org/10.1007/7657_2015_78