@inbook{a672f004443f4951807b25e9e9435de9,
title = "Dual-labeling immuno-electron microscopic method for identifying pre- and postsynaptic profiles in Mammalian brain",
abstract = "Mammalian nervous system communicates mainly through chemical synapses with neurotransmitters in the presynaptic axon terminal and their receptors on the postsynaptic dendritic targets. We describe here an immuno-electron microscopic (immuno-EM) method to simultaneously label presynaptic neurotransmitters with postembedding immunogold and postsynaptic components with preembedding immunoperoxidase. This dual-immuno-EM labeling technique allows reliable distinction of the two types of EMsignals in closely related ultrastructures. The key steps of the procedure and the detailed protocol are provided.",
keywords = "Dual labeling, Excitatory neurotransmitter, Gold particles, Immuno-EM, Immunoperoxidase, Postsynaptic density",
author = "Liu, {Xiao Bo} and Cheng, {Hwai Jong}",
year = "2016",
doi = "10.1007/7657_2015_78",
language = "English (US)",
volume = "115",
series = "Neuromethods",
publisher = "Humana Press Inc.",
pages = "21--33",
booktitle = "Neuromethods",
}