Dual congenital transmission of Toxoplasma gondii and Sarcocystis neurona in a late-term aborted pup from a chronically infected southern sea otter (Enhydra lutris nereis)

Karen Shapiro, Melissa A. Miller, Andrea E. Packham, Beatriz Aguilar, Patricia A Conrad, Elizabeth Vanwormer, Michael J. Murray

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Toxoplasma gondii and Sarcocystis neurona are protozoan parasites with terrestrial definitive hosts, and both pathogens can cause fatal disease in a wide range of marine animals. Close monitoring of threatened southern sea otters (Enhydra lutris nereis) in California allowed for the diagnosis of dual transplacental transmission of T. gondii and S. neurona in a wild female otter that was chronically infected with both parasites. Congenital infection resulted in late-term abortion due to disseminated toxoplasmosis. Toxoplasma gondii and S. neurona DNA was amplified from placental tissue culture, as well as from fetal lung tissue. Molecular characterization of T. gondii revealed a Type X genotype in isolates derived from placenta and fetal brain, as well as in all tested fetal organs (brain, lung, spleen, liver and thymus). This report provides the first evidence for transplacental transmission of T. gondii in a chronically infected wild sea otter, and the first molecular and immunohistochemical confirmation of concurrent transplacental transmission of T. gondii and S. neurona in any species. Repeated fetal and/or neonatal losses in the sea otter dam also suggested that T. gondii has the potential to reduce fecundity in chronically infected marine mammals through parasite recrudescence and repeated fetal infection.

Original languageEnglish (US)
Pages (from-to)276-288
Number of pages13
JournalParasitology
Volume143
Issue number3
DOIs
StatePublished - Mar 1 2016

Fingerprint

Otters
Sarcocystis
Sarcocystis neurona
Toxoplasma
Toxoplasma gondii
pups
transplacental transmission
Enhydra lutris
Parasites
parasites
lungs
Dual (Psychiatry) Diagnosis
brain
Lung
abortion (animals)
toxoplasmosis
Toxoplasmosis
Brain
marine mammals
Infection

Keywords

  • abortion
  • congenital transmission
  • Sarcocystis neurona
  • sea otter
  • Toxoplasma gondii
  • transmammary transmission

ASJC Scopus subject areas

  • Parasitology
  • Animal Science and Zoology
  • Infectious Diseases

Cite this

Dual congenital transmission of Toxoplasma gondii and Sarcocystis neurona in a late-term aborted pup from a chronically infected southern sea otter (Enhydra lutris nereis). / Shapiro, Karen; Miller, Melissa A.; Packham, Andrea E.; Aguilar, Beatriz; Conrad, Patricia A; Vanwormer, Elizabeth; Murray, Michael J.

In: Parasitology, Vol. 143, No. 3, 01.03.2016, p. 276-288.

Research output: Contribution to journalArticle

Shapiro, Karen ; Miller, Melissa A. ; Packham, Andrea E. ; Aguilar, Beatriz ; Conrad, Patricia A ; Vanwormer, Elizabeth ; Murray, Michael J. / Dual congenital transmission of Toxoplasma gondii and Sarcocystis neurona in a late-term aborted pup from a chronically infected southern sea otter (Enhydra lutris nereis). In: Parasitology. 2016 ; Vol. 143, No. 3. pp. 276-288.
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