Droplet digital PCR enabled by microfluidic impact printing for absolute gene quantification

Yang Pan, Tuo Ma, Qi Meng, Yuxin Mao, Kaiqin Chu, Yongfan Men, Tingrui Pan, Baoqing Li, Jiaru Chu

Research output: Contribution to journalArticle

Abstract

Digital PCR enabled high-sensitivity and quantitative measurements of rare biological variants. A new digital droplet-enabled PCR technology was introduced in this paper, which partitioned genetic targets into a planar nanoliter droplet array by using a microfluidic impact printer (MIP) with a disposable microfluidic chip. The accuracy of this MIP-enabled PCR technology was verified by detecting a series of concentration gradients of GAPDH gene across spanning four orders of magnitude (from 0.464 copies/μL to 464 copies/μL). Furthermore, this technology was applied to detect the expressions of p53 gene in colon cancer tissues and adjacent nontumorous tissues, from which the copies of the nucleic acids could be absolute-quantitatively determined. The outcomes were consistent with the results of using the conventional real-time PCR, demonstrating a great potential of the MIP-enabled digital PCR in detecting gene expression in clinical samples.

Original languageEnglish (US)
Article number120680
JournalTalanta
Volume211
DOIs
StatePublished - May 1 2020

Keywords

  • Digital PCR
  • Genetic analyses
  • Microfluidic impact printing

ASJC Scopus subject areas

  • Analytical Chemistry

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