Drinking water source and reproductive outcomes in Sprague-Dawley rats

Janet Y. Uriu-Hare, Shanna H. Swan, Linh M. Bui, Raymond R. Neutra, Carl L Keen

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

As part of our work on the influence of water source on reproductive outcome, Sprague-Dawley rats were randomized to tap water, bottled water, or deionized water treatment groups, utilizing 160 animals per treatment; animals received the water prior to and during pregnancy. Rats were shipped in four batches (A-D). Batch effects were seen for several reproductive parameters. Because the tap water supply was interrupted by an earthquake resulting in an unbalanced design, primary analyses utilized only batches C and D, which included most of the tap water-treated rats. A treatment effect with respect to resorption frequency was seen that was marginally significant using a fixed-effects analysis of variance (P = 0.053), but not when batch was entered as a random effect (P = 0.36). The data were modeled by logistic regression, controlling for batch, litter size, and batch-treatment interaction. The odds ratio comparing tap to bottled water was 1.8 (95% CI 1.0 to 3.3, P = 0.05), which was similar to the epidemiologic result that prompted this study. The magnitude of this association varied by batch, and the difference in resorption frequency was within the range of variation seen for control animals. Although these findings do not justify public health action at this time, further investigation is warranted.

Original languageEnglish (US)
Pages (from-to)549-561
Number of pages13
JournalReproductive Toxicology
Volume9
Issue number6
DOIs
StatePublished - 1995

Fingerprint

Drinking Water
Sprague Dawley Rats
Rats
Animals
Water
Earthquakes
Litter Size
Water Purification
Water Supply
Deionized water
Public health
Analysis of variance (ANOVA)
Water treatment
Water supply
Logistics
Analysis of Variance
Public Health
Logistic Models
Odds Ratio
Pregnancy

Keywords

  • bacteria
  • pregnancy outcome
  • rats
  • reproduction
  • resorption
  • water

ASJC Scopus subject areas

  • Toxicology

Cite this

Uriu-Hare, J. Y., Swan, S. H., Bui, L. M., Neutra, R. R., & Keen, C. L. (1995). Drinking water source and reproductive outcomes in Sprague-Dawley rats. Reproductive Toxicology, 9(6), 549-561. https://doi.org/10.1016/0890-6238(95)02005-5

Drinking water source and reproductive outcomes in Sprague-Dawley rats. / Uriu-Hare, Janet Y.; Swan, Shanna H.; Bui, Linh M.; Neutra, Raymond R.; Keen, Carl L.

In: Reproductive Toxicology, Vol. 9, No. 6, 1995, p. 549-561.

Research output: Contribution to journalArticle

Uriu-Hare, JY, Swan, SH, Bui, LM, Neutra, RR & Keen, CL 1995, 'Drinking water source and reproductive outcomes in Sprague-Dawley rats', Reproductive Toxicology, vol. 9, no. 6, pp. 549-561. https://doi.org/10.1016/0890-6238(95)02005-5
Uriu-Hare, Janet Y. ; Swan, Shanna H. ; Bui, Linh M. ; Neutra, Raymond R. ; Keen, Carl L. / Drinking water source and reproductive outcomes in Sprague-Dawley rats. In: Reproductive Toxicology. 1995 ; Vol. 9, No. 6. pp. 549-561.
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