Downregulation of NF-κB activity associated with alteration in proliferative response in the spleen after burn injury

Ho H. Phan, Kiho Cho, Heather A. Nelson, Soowan Shin, Jayoung Jeong, David G. Greenhalgh

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Alterations in proliferation status and cellular composition of immune organs are among key events in the modulation of immune function after burn injury. Nuclear factor (NF)-κB is a transcription factor that plays a pivotal role in the response to injury as well as immune cell differentiation and proliferation. In this study, we investigated the effects of burn injury on the activity of NF-κB and its association with cellular proliferation in the spleen. Western analysis of whole spleen tissues of mice after 18% burn injury revealed a marked reduction in nuclear NF-κB rel A protein expression 3 to 21 days after injury when there was an increase in proliferative activity in the red pulp of the spleen after injury as indicated by an increase in proliferating cell nuclear antigen (PCNA). In the splenic B cells, however, the down-regulation of NF-κB rel A was associated with decreased PCNA expression as well as IκBA and phosphorylated IκBα. In contrast, no significant change in NF-κB rel A or PCNA expression was observed for splenic T cells. These data suggest that there is a differential regulation of NF-κB and proliferative activity in the splenic cell subsets after burn injury. Furthermore, the regulation of NF-κB may be linked to the proliferative changes seen in the spleen after burn injury.

Original languageEnglish (US)
Pages (from-to)73-79
Number of pages7
JournalShock
Volume23
Issue number1
DOIs
StatePublished - Jan 2005

Keywords

  • B cells
  • IκBα
  • Immune
  • PCNA
  • rel A (p65)
  • T cells
  • Thermal

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Physiology

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