Double-labeled immunofluorescence study of cutaneous nerves in psoriasis

Wen Yue Jiang, Siba P Raychaudhuri, Eugene M. Farber

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Background and objective: In recent years, many reports have suggested an active role of neuropeptides in the pathogenesis of psoriasis. Increased numbers of neuropeptidecontaining nerves positive for substance P (SP), vasoactive intestinal polypeptide (VIP), and calcium gene-related peptide (CGRP) have been reported in psoriatic tissue. As psoriatic epidermis has a larger mass/volume, however, it is expected to have more nerves and a higher number of neuropeptergic fibers. Therefore, instead of demonstrating a larger number of neuropeptergic fibers, a more significant study is to investigate whether the neuropeptergic fibers are denser in psoriatic tissue. In this study, we applied a double labeled immunofluorescence technique. This method allows the identification of the total number of nerve fibers and the number of nerves positive for specific neuropeptides. Materials and methods: We obtained biopsies from nine lesional and seven non-lesional psoriatic skins and six normal controls. Biopsies were snap frozen and then cut into 14 μm cryosections. The tissues were first treated with anti-microtubule associated protein (MAP)2 antibody to stain the nerves. This was followed by a second set of stainings for SP, VIP, and CGRP. Primary antibodies were used in dilutions of 1: 200 for anti-MAP2, 1 : 200 for anti-SP, 1 : 800 for anti- VIP, and 1 : 400 for anti-CGRP. Results We found that the percentage of SP- positive fibers was twofold greater and the percentage of CGRP-positive fibers was 2.5 times greater in the psoriatic epidermis than in the epidermis of normal skin. Psoriatic epidermis had 30.1 ± 3.9% SP-positive nerve fibers compared with 15.7 ± 3.7% in the normal control. The corresponding values for CGRP-positive nerve fibers were 30.1 ± 3.9% and 12.0 ± 4.2%. Conclusions: The results of our study suggest that SP- and CGRP-containing neuropeptide nerve fibers are more dense in the psoriatic epidermis. Both SP and CGRP are chemotactic to neutrophils and mitogenic to keratinocytes and endothelial cells. In addition, SP activates T lymphocytes and induces adhesion molecules on the endothelial cells. Our observations suggest that neuropeptides may play a significant role in the inflammatory and proliferative process of psoriasis.

Original languageEnglish (US)
Pages (from-to)572-574
Number of pages3
JournalInternational Journal of Dermatology
Volume37
Issue number8
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

Substance P
Psoriasis
Fluorescent Antibody Technique
Skin
Epidermis
Calcium
Peptides
Neuropeptides
Nerve Fibers
Vasoactive Intestinal Peptide
Genes
Endothelial Cells
Biopsy
Microtubule-Associated Proteins
Antibodies
Keratinocytes
Neutrophils
Coloring Agents
Staining and Labeling
T-Lymphocytes

ASJC Scopus subject areas

  • Dermatology

Cite this

Double-labeled immunofluorescence study of cutaneous nerves in psoriasis. / Jiang, Wen Yue; Raychaudhuri, Siba P; Farber, Eugene M.

In: International Journal of Dermatology, Vol. 37, No. 8, 1998, p. 572-574.

Research output: Contribution to journalArticle

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title = "Double-labeled immunofluorescence study of cutaneous nerves in psoriasis",
abstract = "Background and objective: In recent years, many reports have suggested an active role of neuropeptides in the pathogenesis of psoriasis. Increased numbers of neuropeptidecontaining nerves positive for substance P (SP), vasoactive intestinal polypeptide (VIP), and calcium gene-related peptide (CGRP) have been reported in psoriatic tissue. As psoriatic epidermis has a larger mass/volume, however, it is expected to have more nerves and a higher number of neuropeptergic fibers. Therefore, instead of demonstrating a larger number of neuropeptergic fibers, a more significant study is to investigate whether the neuropeptergic fibers are denser in psoriatic tissue. In this study, we applied a double labeled immunofluorescence technique. This method allows the identification of the total number of nerve fibers and the number of nerves positive for specific neuropeptides. Materials and methods: We obtained biopsies from nine lesional and seven non-lesional psoriatic skins and six normal controls. Biopsies were snap frozen and then cut into 14 μm cryosections. The tissues were first treated with anti-microtubule associated protein (MAP)2 antibody to stain the nerves. This was followed by a second set of stainings for SP, VIP, and CGRP. Primary antibodies were used in dilutions of 1: 200 for anti-MAP2, 1 : 200 for anti-SP, 1 : 800 for anti- VIP, and 1 : 400 for anti-CGRP. Results We found that the percentage of SP- positive fibers was twofold greater and the percentage of CGRP-positive fibers was 2.5 times greater in the psoriatic epidermis than in the epidermis of normal skin. Psoriatic epidermis had 30.1 ± 3.9{\%} SP-positive nerve fibers compared with 15.7 ± 3.7{\%} in the normal control. The corresponding values for CGRP-positive nerve fibers were 30.1 ± 3.9{\%} and 12.0 ± 4.2{\%}. Conclusions: The results of our study suggest that SP- and CGRP-containing neuropeptide nerve fibers are more dense in the psoriatic epidermis. Both SP and CGRP are chemotactic to neutrophils and mitogenic to keratinocytes and endothelial cells. In addition, SP activates T lymphocytes and induces adhesion molecules on the endothelial cells. Our observations suggest that neuropeptides may play a significant role in the inflammatory and proliferative process of psoriasis.",
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T1 - Double-labeled immunofluorescence study of cutaneous nerves in psoriasis

AU - Jiang, Wen Yue

AU - Raychaudhuri, Siba P

AU - Farber, Eugene M.

PY - 1998

Y1 - 1998

N2 - Background and objective: In recent years, many reports have suggested an active role of neuropeptides in the pathogenesis of psoriasis. Increased numbers of neuropeptidecontaining nerves positive for substance P (SP), vasoactive intestinal polypeptide (VIP), and calcium gene-related peptide (CGRP) have been reported in psoriatic tissue. As psoriatic epidermis has a larger mass/volume, however, it is expected to have more nerves and a higher number of neuropeptergic fibers. Therefore, instead of demonstrating a larger number of neuropeptergic fibers, a more significant study is to investigate whether the neuropeptergic fibers are denser in psoriatic tissue. In this study, we applied a double labeled immunofluorescence technique. This method allows the identification of the total number of nerve fibers and the number of nerves positive for specific neuropeptides. Materials and methods: We obtained biopsies from nine lesional and seven non-lesional psoriatic skins and six normal controls. Biopsies were snap frozen and then cut into 14 μm cryosections. The tissues were first treated with anti-microtubule associated protein (MAP)2 antibody to stain the nerves. This was followed by a second set of stainings for SP, VIP, and CGRP. Primary antibodies were used in dilutions of 1: 200 for anti-MAP2, 1 : 200 for anti-SP, 1 : 800 for anti- VIP, and 1 : 400 for anti-CGRP. Results We found that the percentage of SP- positive fibers was twofold greater and the percentage of CGRP-positive fibers was 2.5 times greater in the psoriatic epidermis than in the epidermis of normal skin. Psoriatic epidermis had 30.1 ± 3.9% SP-positive nerve fibers compared with 15.7 ± 3.7% in the normal control. The corresponding values for CGRP-positive nerve fibers were 30.1 ± 3.9% and 12.0 ± 4.2%. Conclusions: The results of our study suggest that SP- and CGRP-containing neuropeptide nerve fibers are more dense in the psoriatic epidermis. Both SP and CGRP are chemotactic to neutrophils and mitogenic to keratinocytes and endothelial cells. In addition, SP activates T lymphocytes and induces adhesion molecules on the endothelial cells. Our observations suggest that neuropeptides may play a significant role in the inflammatory and proliferative process of psoriasis.

AB - Background and objective: In recent years, many reports have suggested an active role of neuropeptides in the pathogenesis of psoriasis. Increased numbers of neuropeptidecontaining nerves positive for substance P (SP), vasoactive intestinal polypeptide (VIP), and calcium gene-related peptide (CGRP) have been reported in psoriatic tissue. As psoriatic epidermis has a larger mass/volume, however, it is expected to have more nerves and a higher number of neuropeptergic fibers. Therefore, instead of demonstrating a larger number of neuropeptergic fibers, a more significant study is to investigate whether the neuropeptergic fibers are denser in psoriatic tissue. In this study, we applied a double labeled immunofluorescence technique. This method allows the identification of the total number of nerve fibers and the number of nerves positive for specific neuropeptides. Materials and methods: We obtained biopsies from nine lesional and seven non-lesional psoriatic skins and six normal controls. Biopsies were snap frozen and then cut into 14 μm cryosections. The tissues were first treated with anti-microtubule associated protein (MAP)2 antibody to stain the nerves. This was followed by a second set of stainings for SP, VIP, and CGRP. Primary antibodies were used in dilutions of 1: 200 for anti-MAP2, 1 : 200 for anti-SP, 1 : 800 for anti- VIP, and 1 : 400 for anti-CGRP. Results We found that the percentage of SP- positive fibers was twofold greater and the percentage of CGRP-positive fibers was 2.5 times greater in the psoriatic epidermis than in the epidermis of normal skin. Psoriatic epidermis had 30.1 ± 3.9% SP-positive nerve fibers compared with 15.7 ± 3.7% in the normal control. The corresponding values for CGRP-positive nerve fibers were 30.1 ± 3.9% and 12.0 ± 4.2%. Conclusions: The results of our study suggest that SP- and CGRP-containing neuropeptide nerve fibers are more dense in the psoriatic epidermis. Both SP and CGRP are chemotactic to neutrophils and mitogenic to keratinocytes and endothelial cells. In addition, SP activates T lymphocytes and induces adhesion molecules on the endothelial cells. Our observations suggest that neuropeptides may play a significant role in the inflammatory and proliferative process of psoriasis.

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