Dotette: Programmable, high-precision, plug-and-play droplet pipetting

Jinzhen Fan, Yongfan Men, Kuo Hao Tseng, Yi Ding, Yunfeng Ding, Fernando Villarreal, Cheemeng Tan, Baoqing Li, Tingrui Pan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Manual micropipettes are the most heavily used liquid handling devices in biological and chemical laboratories; however, they suffer from low precision for volumes under 1 μl and inevitable human errors. For a manual device, the human errors introduced pose potential risks of failed experiments, inaccurate results, and financial costs. Meanwhile, low precision under 1 μl can cause severe quantification errors and high heterogeneity of outcomes, becoming a bottleneck of reaction miniaturization for quantitative research in biochemical labs. Here, we report Dotette, a programmable, plug-and-play microfluidic pipetting device based on nanoliter liquid printing. With automated control, protocols designed on computers can be directly downloaded into Dotette, enabling programmable operation processes. Utilizing continuous nanoliter droplet dispensing, the precision of the volume control has been successfully improved from traditional 20%-50% to less than 5% in the range of 100 nl to 1000 nl. Such a highly automated, plug-and-play add-on to existing pipetting devices not only improves precise quantification in low-volume liquid handling and reduces chemical consumptions but also facilitates and automates a variety of biochemical and biological operations.

Original languageEnglish (US)
Article number034107
JournalBiomicrofluidics
Volume12
Issue number3
DOIs
StatePublished - May 1 2018

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plugs
Lab-On-A-Chip Devices
Equipment and Supplies
Liquids
liquids
Chemical laboratories
Laboratory Chemicals
Miniaturization
Printing
microfluidic devices
miniaturization
Microfluidics
printing
costs
Costs and Cost Analysis
causes
Research
Costs
Experiments

ASJC Scopus subject areas

  • Molecular Biology
  • Materials Science(all)
  • Genetics
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

Cite this

Fan, J., Men, Y., Hao Tseng, K., Ding, Y., Ding, Y., Villarreal, F., ... Pan, T. (2018). Dotette: Programmable, high-precision, plug-and-play droplet pipetting. Biomicrofluidics, 12(3), [034107]. https://doi.org/10.1063/1.5030629

Dotette : Programmable, high-precision, plug-and-play droplet pipetting. / Fan, Jinzhen; Men, Yongfan; Hao Tseng, Kuo; Ding, Yi; Ding, Yunfeng; Villarreal, Fernando; Tan, Cheemeng; Li, Baoqing; Pan, Tingrui.

In: Biomicrofluidics, Vol. 12, No. 3, 034107, 01.05.2018.

Research output: Contribution to journalArticle

Fan, J, Men, Y, Hao Tseng, K, Ding, Y, Ding, Y, Villarreal, F, Tan, C, Li, B & Pan, T 2018, 'Dotette: Programmable, high-precision, plug-and-play droplet pipetting', Biomicrofluidics, vol. 12, no. 3, 034107. https://doi.org/10.1063/1.5030629
Fan J, Men Y, Hao Tseng K, Ding Y, Ding Y, Villarreal F et al. Dotette: Programmable, high-precision, plug-and-play droplet pipetting. Biomicrofluidics. 2018 May 1;12(3). 034107. https://doi.org/10.1063/1.5030629
Fan, Jinzhen ; Men, Yongfan ; Hao Tseng, Kuo ; Ding, Yi ; Ding, Yunfeng ; Villarreal, Fernando ; Tan, Cheemeng ; Li, Baoqing ; Pan, Tingrui. / Dotette : Programmable, high-precision, plug-and-play droplet pipetting. In: Biomicrofluidics. 2018 ; Vol. 12, No. 3.
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