Dosimetry of N6-formyllysine adducts following [ 13C2H2]-formaldehyde exposures in rats

Bahar Edrissi, Koli Taghizadeh, Benjamin Moeller, Dean Kracko, Melanie Doyle-Eisele, James A. Swenberg, Peter C. Dedon

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

With formaldehyde as the major source of endogenous N6- formyllysine protein adducts, we quantified endogenous and exogenous N 6-formyllysine in the nasal epithelium of rats exposed by inhalation to 0.7, 2, 5.8, and 9.1 ppm [13C2H2]- formaldehyde using liquid chromatography-coupled tandem mass spectrometry. Exogenous N6-formyllysine was detected in the nasal epithelium, with concentration-dependent formation in total as well as fractionated (cytoplasmic, membrane, nuclear) proteins, but was not detected in the lung, liver, or bone marrow. Endogenous adducts dominated at all exposure conditions, with a 6 h 9.1 ppm formaldehyde exposure resulting in one-third of the total load of N 6-formyllysine being derived from exogenous sources. The results parallel previous studies of formaldehyde-induced DNA adducts.

Original languageEnglish (US)
Pages (from-to)1421-1423
Number of pages3
JournalChemical Research in Toxicology
Volume26
Issue number10
DOIs
StatePublished - Nov 4 2013
Externally publishedYes

ASJC Scopus subject areas

  • Toxicology

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