Dorsal horn neurons expressing NK-1 receptors mediate scratching in rats

Earl Carstens, Mirela Iodi Carstens, Christopher T. Simons, Steven L. Jinks

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Itch is thought to be signaled by pruritogen-responsive neurons in the superficial spinal dorsal horn. Many neurons here express the substance P NK-1 receptor. We investigated whether neurotoxic destruction of spinal NK-1-expressing neurons affected itch-related scratching behavior. Rats received intracisternal substance P conjugated to saporin (SP-SAP), or saporin (SAP) only (controls), and were subsequently tested for scratching behavior elicited by intradermal 5-hydroxytryptamine. SAP controls exhibited dose-related hindlimb scratching, which was significantly attenuated in SP-SAP-treated rats. There was a virtual absence of NK-1 immunoreactive neurons in superficial laminae of the upper cervical and medullary dorsal horn in SP-SAP-treated rats. These results indicate that superficial dorsal horn neurons expressing NK-1 receptors play a key role in spinal itch transmission.

Original languageEnglish (US)
Pages (from-to)303-308
Number of pages6
JournalNeuroReport
Volume21
Issue number4
DOIs
StatePublished - Mar 2010

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Posterior Horn Cells
Neurokinin-1 Receptors
Neurons
Substance P
Hindlimb
saporin
Serotonin

Keywords

  • Itch
  • Scratching
  • Serotonin
  • Substance P-saporin
  • Superficial dorsal horn

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Carstens, E., Carstens, M. I., Simons, C. T., & Jinks, S. L. (2010). Dorsal horn neurons expressing NK-1 receptors mediate scratching in rats. NeuroReport, 21(4), 303-308. https://doi.org/10.1097/WNR.0b013e328337310a

Dorsal horn neurons expressing NK-1 receptors mediate scratching in rats. / Carstens, Earl; Carstens, Mirela Iodi; Simons, Christopher T.; Jinks, Steven L.

In: NeuroReport, Vol. 21, No. 4, 03.2010, p. 303-308.

Research output: Contribution to journalArticle

Carstens, E, Carstens, MI, Simons, CT & Jinks, SL 2010, 'Dorsal horn neurons expressing NK-1 receptors mediate scratching in rats', NeuroReport, vol. 21, no. 4, pp. 303-308. https://doi.org/10.1097/WNR.0b013e328337310a
Carstens, Earl ; Carstens, Mirela Iodi ; Simons, Christopher T. ; Jinks, Steven L. / Dorsal horn neurons expressing NK-1 receptors mediate scratching in rats. In: NeuroReport. 2010 ; Vol. 21, No. 4. pp. 303-308.
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