Imprinting marks the parental origin of chromosomes, resulting in allele- specific changes in chromatin organization, transcription and replication. We report a 50-60 kb domain of allele-specific replication between the γ- aminobutyric acid receptor subunit β3 (GABRB3) and α5 (GABRA5) genes. Replication of this domain occurs in early S phase on the maternal chromosome 15 but is delayed until the end of S phase on the paternal homologue. In contrast, the genomic regions flanking this domain exhibit paternal earlier replication in mid to late S phase. Uniparental disomy or hemizygous deletion of chromosome 15 results in altered allele-specific replication kinetics compared with normals, suggesting that allele-specific replication within the GABRB3/A5 region may be regulated by reciprocal imprints on the maternal and paternal chromosomes.
|Original language||English (US)|
|Number of pages||9|
|State||Published - 1995|
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