Does training affect mRNA transciption for cytokine production in circulating leucocytes?

P. T. Colahan, C. Kollias-Bakert, C. M. Leutenegger, James H Jones

Research output: Chapter in Book/Report/Conference proceedingChapter

22 Citations (Scopus)

Abstract

Physical exertion is a stimulus for the upregulation of cytokine production including IL-1beta, IL-1ra, IL-2, IL-4, IL-6, IL-10 and TNF-alpha in horses. To investigate that hypothesis, we initiated training of 5 stall-rested Thoroughbreds. Blood samples were drawn before and weekly during training. The relative transcription of mRNA within the leucocytes was measured using real time TaqMan quantitative PCR. The training protocol was walking (3 min), trotting (3 min) and cantering/galloping (6 min) increasing in intensity weekly (6 to 12 m/s) and culminating in an intense exercise period. Comparisons of mRNA concentrations were made using a repeated measures ANOVA on ranks and a Student-Newman-Keuls pair-wise multiple comparison (P<0.05). The training programme or intense exercise bout did not affect IL-2, IL-4, IL-6 and IL-10. IL1-beta and TNF-alpha transcription increased on Day 23. TNF-alpha peaked on Day 23 and IL1-beta on Day 30. Neither demonstrated a response to intense exercise. IL-1ra decreased significantly on Day 9; rose significantly from Day 9 to Days 16 and 23; remained significantly elevated through Days 30 and 37 over Day 9, and rose very slightly after intense exercise on Day 56. Alterations in leukocyte cytokine responses may influence susceptibility to infectious disease, metabolic responses to exercise or exercised induced syndromes.

Original languageEnglish (US)
Title of host publicationEquine veterinary journal. Supplement
Pages154-158
Number of pages5
Edition34
StatePublished - 2002
Externally publishedYes

Fingerprint

Leukocytes
Exercise
Cytokines
Messenger RNA
Interleukin 1 Receptor Antagonist Protein
Tumor Necrosis Factor-alpha
Interleukin-4
Interleukin-10
Interleukin-2
Interleukin-6
Physical Exertion
Horses
Walking
Communicable Diseases
Real-Time Polymerase Chain Reaction
Analysis of Variance
Up-Regulation
Students
Education

Cite this

Colahan, P. T., Kollias-Bakert, C., Leutenegger, C. M., & Jones, J. H. (2002). Does training affect mRNA transciption for cytokine production in circulating leucocytes? In Equine veterinary journal. Supplement (34 ed., pp. 154-158)

Does training affect mRNA transciption for cytokine production in circulating leucocytes? / Colahan, P. T.; Kollias-Bakert, C.; Leutenegger, C. M.; Jones, James H.

Equine veterinary journal. Supplement. 34. ed. 2002. p. 154-158.

Research output: Chapter in Book/Report/Conference proceedingChapter

Colahan, PT, Kollias-Bakert, C, Leutenegger, CM & Jones, JH 2002, Does training affect mRNA transciption for cytokine production in circulating leucocytes? in Equine veterinary journal. Supplement. 34 edn, pp. 154-158.
Colahan PT, Kollias-Bakert C, Leutenegger CM, Jones JH. Does training affect mRNA transciption for cytokine production in circulating leucocytes? In Equine veterinary journal. Supplement. 34 ed. 2002. p. 154-158
Colahan, P. T. ; Kollias-Bakert, C. ; Leutenegger, C. M. ; Jones, James H. / Does training affect mRNA transciption for cytokine production in circulating leucocytes?. Equine veterinary journal. Supplement. 34. ed. 2002. pp. 154-158
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