Does lead interfere with hemoglobin-based oxygen carrier (HBOC) function? A pilot study of lead concentrations in three approved or tested HBOCs and oxyhemoglobin dissociation with HBOCs and/or bovine blood with varying lead concentrations

Ahsanul K. Khan, Jonathan S. Jahr, Susmita Nesargi, Stephen J. Rothenberg, Zuping Tang, Anthony Cheung, Robert A. Gunther, Gerald J Kost, Bernd Driessen

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Abstract

We measured lead concentrations in three hemoglobin-based oxygen carriers (HBOCs; Oxyglobin®, Hemopure®, and Hemolink™) and compared them with lead concentrations from blood-bank blood. Oxyhemoglobin dissociation was measured with large concentrations of lead in bovine HBOC, with or without bovine blood, and in bovine blood. Samples of each were prepared by combining one with normal saline (control), the second with small lead concentrations (22 μg/dL), and the third with toxic lead concentrations (70 μg/dL). They were blended in 2 tonometers at oxygen concentrations (2.5%, 5%, 8%, 10%, 21%, and 95%) with 5% CO2 and the remainder nitrogen for 5 min per sample after a 15-min wash-in with each level of oxygen and were measured with co-oximetry. Oxygen saturation was plotted against Po2, fitting fourth-order polynomial nonlinear regression to the data. The lead concentrations of the three HBOCs were 0.51, 0.22, 0.40 μg/dL. There were no clinically important differences of the oxyhemoglobin dissociation curves as a function of lead concentration. The lead concentrations of the three tested HBOCs were small and no larger than the average for blood-bank blood. The presence of increasing concentrations of lead in either concentrated solution of bovine HBOC or a 1:1 mixture of bovine HBOC and native bovine blood does not appear to affect hemoglobin oxygenation in an acute in vitro model of increased lead concentrations.

Original languageEnglish (US)
Pages (from-to)1813-1820
Number of pages8
JournalAnesthesia and Analgesia
Volume96
Issue number6
StatePublished - Jun 1 2003

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Oxyhemoglobins
Hemoglobins
Oxygen
Blood Banks
Oximetry
Poisons
Lead
Nitrogen

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

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Does lead interfere with hemoglobin-based oxygen carrier (HBOC) function? A pilot study of lead concentrations in three approved or tested HBOCs and oxyhemoglobin dissociation with HBOCs and/or bovine blood with varying lead concentrations. / Khan, Ahsanul K.; Jahr, Jonathan S.; Nesargi, Susmita; Rothenberg, Stephen J.; Tang, Zuping; Cheung, Anthony; Gunther, Robert A.; Kost, Gerald J; Driessen, Bernd.

In: Anesthesia and Analgesia, Vol. 96, No. 6, 01.06.2003, p. 1813-1820.

Research output: Contribution to journalArticle

Khan, Ahsanul K. ; Jahr, Jonathan S. ; Nesargi, Susmita ; Rothenberg, Stephen J. ; Tang, Zuping ; Cheung, Anthony ; Gunther, Robert A. ; Kost, Gerald J ; Driessen, Bernd. / Does lead interfere with hemoglobin-based oxygen carrier (HBOC) function? A pilot study of lead concentrations in three approved or tested HBOCs and oxyhemoglobin dissociation with HBOCs and/or bovine blood with varying lead concentrations. In: Anesthesia and Analgesia. 2003 ; Vol. 96, No. 6. pp. 1813-1820.
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