DNA vaccination of mice to immunize against Coccidioides immitis

E. M. Siegel, L. Shubitz, C. R. Zimmermann, K. I. Orsborn, D. Pappagianis, C. R. Sterling, J. N. Galgiani

Research output: Contribution to journalArticle

Abstract

Life-long immunity follows most coccidioidal infections and vaccination with killed spherule vaccine protects mice from later infection. Indirect evidence implicates a proline-rich antigen (PRA) as a stimulus of cellular immunity. To study this directly, a 597 bp PCR amplimer encoding PRA was inserted into plasmid vectors VR1012 and VR1020 (Vical, San Diego CA). DNA sequencing verified orientation and reading frame. Female Swiss-Webster mice, 6-8 weeks old, received 100 μg i.m. of plasmid DNA. ELISA with recombinant PRA was used to detect a humoral response. After a single vaccination with either vector, PRA-specific IgG was detected by 2 wks (mean±SEM OD of 0.347±0.038 versus 0.128±0.004 for vector control mice, serum diluted 1:320, p<0.004). Antibodies were elevated for over 2 months. Vaccination repeated at 4 wks boosted IgG levels in 6 of 7 mice studied. In a preliminary study of intranasal C. immitis infection, 8 surviving mice had higher preinfection IgG levels in serum diluted 1:640 (0.939 ± 0.162) as compared to 4 mice who died before day 21 (0.494 ± 0.167). DNA vaccination will be a useful tool for studies of coccidioidal immunity.

Original languageEnglish (US)
Pages (from-to)357
Number of pages1
JournalClinical Infectious Diseases
Volume25
Issue number2
StatePublished - 1997

ASJC Scopus subject areas

  • Immunology

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    Siegel, E. M., Shubitz, L., Zimmermann, C. R., Orsborn, K. I., Pappagianis, D., Sterling, C. R., & Galgiani, J. N. (1997). DNA vaccination of mice to immunize against Coccidioides immitis. Clinical Infectious Diseases, 25(2), 357.