DNA topoisomerase II is required for condensation and separation of mitotic chromosomes in S. pombe

Tadashi Uemura, Hiroyuki Ohkura, Yasuhisa Adachi, Kazuhiko Morino, Kazuhiro Shiozaki, Mitsuhiro Yanagida

Research output: Contribution to journalArticle

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Abstract

We show that DNA topoisomerase II (topo II) is continuously required for mitotic chromosome changes in Schizosaccharomyces pombe. We constructed cold-sensitive (cs) or temperature-sensitive (ts) strains mutated in the genes coding for topo II (top2) and β-tubulin (nda3). The ATP-dependent activity of the top2cs gene product is cs in vitro. The cloned top2cs gene sequence predicts an amino acid substitution. A cs top2-cs nda3 double mutant at 20°C shows long, entangled chromosomes, which condense and separate upon the shift to permissive temperatures. If spindle formation is prevented at permissive temperatures, the chromosomes condense but do not separate. Thus topo II is required for final chromosome condensation; moreover, pulse-shift experiments show that topo II is required for chromatid disjuction. Experiments with ts top2-cs nda3 cells show that topo II is also required for chromosome separation in anaphase: inactivation of topo II and activation of β-tubulin allow normal spindle formation but result in "streaked" chromosomes.

Original languageEnglish (US)
Pages (from-to)917-925
Number of pages9
JournalCell
Volume50
Issue number6
DOIs
StatePublished - Sep 11 1987
Externally publishedYes

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Type II DNA Topoisomerase
Schizosaccharomyces
Chromosomes
Condensation
Temperature
Genes
Tubulin
Anaphase
Chromatids
Amino Acid Substitution
Substitution reactions
Adenosine Triphosphate
Experiments
Chemical activation
Amino Acids

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Uemura, T., Ohkura, H., Adachi, Y., Morino, K., Shiozaki, K., & Yanagida, M. (1987). DNA topoisomerase II is required for condensation and separation of mitotic chromosomes in S. pombe. Cell, 50(6), 917-925. https://doi.org/10.1016/0092-8674(87)90518-6

DNA topoisomerase II is required for condensation and separation of mitotic chromosomes in S. pombe. / Uemura, Tadashi; Ohkura, Hiroyuki; Adachi, Yasuhisa; Morino, Kazuhiko; Shiozaki, Kazuhiro; Yanagida, Mitsuhiro.

In: Cell, Vol. 50, No. 6, 11.09.1987, p. 917-925.

Research output: Contribution to journalArticle

Uemura, T, Ohkura, H, Adachi, Y, Morino, K, Shiozaki, K & Yanagida, M 1987, 'DNA topoisomerase II is required for condensation and separation of mitotic chromosomes in S. pombe', Cell, vol. 50, no. 6, pp. 917-925. https://doi.org/10.1016/0092-8674(87)90518-6
Uemura, Tadashi ; Ohkura, Hiroyuki ; Adachi, Yasuhisa ; Morino, Kazuhiko ; Shiozaki, Kazuhiro ; Yanagida, Mitsuhiro. / DNA topoisomerase II is required for condensation and separation of mitotic chromosomes in S. pombe. In: Cell. 1987 ; Vol. 50, No. 6. pp. 917-925.
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