DNA strand exchange proteins: a biochemical and physical comparison.

P. R. Bianco, R. B. Tracy, S. C. Kowalczykowski

Research output: Contribution to journalArticle

322 Citations (Scopus)

Abstract

Homologous genetic recombination is an essential biological process that involves the pairing and exchange of DNA between two homologous chromosomes or DNA molecules. It is of fundamental importance to the preservation of genomic integrity, the production of genetic diversity, and the proper segregation of chromosomes. In Escherichia coli, the RecA protein is essential to recombination, and biochemical analysis demonstrates that it is responsible for the crucial steps of homologous pairing and DNA strand exchange. The presence of RecA-like proteins, or their functional equivalents, in bacteriophage, other eubacteria, archaea, and eukaryotes, confirms that the mechanism of homologous pairing and DNA strand exchange is conserved throughout all forms of life. This review focuses on the biochemical and physical characteristics of DNA strand exchange proteins from three diverse organisms: RecA protein from E. coli, UvsX protein from Bacteriophage T4, and RAD51 protein from Saccharomyces cerevisiae.

Original languageEnglish (US)
JournalFrontiers in bioscience : a journal and virtual library
Volume3
StatePublished - 1998
Externally publishedYes

Fingerprint

Rec A Recombinases
DNA
Chromosomes
Proteins
Escherichia coli
Genetic Recombination
Rad51 Recombinase
Biological Phenomena
Bacteriophage T4
Chromosome Segregation
Bacteriophages
Escherichia coli Proteins
Homologous Recombination
Archaea
Eukaryota
Yeast
Saccharomyces cerevisiae
Bacteria
Molecules

Cite this

DNA strand exchange proteins : a biochemical and physical comparison. / Bianco, P. R.; Tracy, R. B.; Kowalczykowski, S. C.

In: Frontiers in bioscience : a journal and virtual library, Vol. 3, 1998.

Research output: Contribution to journalArticle

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