Abstract
Treatment of human respiratory tract epithelial cells with H2O2 led to concentration-dependent DNA strand breakage that was highly-correlated with multiple chemical modifications of all four DNA bases, suggesting that damage is due to hydroxyl radical, OH.. However, the major base damage occurred to adenine. Hence, conclusions made about the occurrence and the extent of oxidative DNA damage on the basis only of changes in 8-hydroxyguanine should be approached with caution.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 233-236 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 374 |
| Issue number | 2 |
| DOIs | |
| State | Published - Oct 30 1995 |
Keywords
- DNA base modification
- DNA damage
- GC-MS
- HO
- Human respiratory tract epithelial cell
- Strand breakage
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Genetics
- Molecular Biology
- Structural Biology
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Cite this
Spencer, J. P. E., Jenner, A., Chimel, K., Aruoma, O. I., Cross, C. E., Wu, R., & Halliwell, B. (1995). DNA strand breakage and base modification induced by hydrogen peroxide treatment of human respiratory tract epithelial cells. FEBS Letters, 374(2), 233-236. https://doi.org/10.1016/0014-5793(95)01117-W