DNA strand breakage and base modification induced by hydrogen peroxide treatment of human respiratory tract epithelial cells

Jeremy P E Spencer; Andrew Jenner; Ken Chimel; Okezie I. Aruoma; Carroll E Cross; Reen Wu; Barry Halliwell

doi:10.1016/0014-5793(95)01117-W

DNA strand breakage and base modification induced by hydrogen peroxide treatment of human respiratory tract epithelial cells

Jeremy P E Spencer, Andrew Jenner, Ken Chimel, Okezie I. Aruoma, Carroll E Cross, Reen Wu, Barry Halliwell

Pulmonary, Critical Care, and Sleep Medicine

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

Treatment of human respiratory tract epithelial cells with H₂O₂ led to concentration-dependent DNA strand breakage that was highly-correlated with multiple chemical modifications of all four DNA bases, suggesting that damage is due to hydroxyl radical, OH^.. However, the major base damage occurred to adenine. Hence, conclusions made about the occurrence and the extent of oxidative DNA damage on the basis only of changes in 8-hydroxyguanine should be approached with caution.

Original language	English (US)
Pages (from-to)	233-236
Number of pages	4
Journal	FEBS Letters
Volume	374
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(95)01117-W
State	Published - Oct 30 1995

Keywords

DNA base modification
DNA damage
GC-MS
HO
Human respiratory tract epithelial cell
Strand breakage

ASJC Scopus subject areas

Biochemistry
Biophysics
Cell Biology
Genetics
Molecular Biology
Structural Biology

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10.1016/0014-5793(95)01117-W

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title = "DNA strand breakage and base modification induced by hydrogen peroxide treatment of human respiratory tract epithelial cells",

abstract = "Treatment of human respiratory tract epithelial cells with H2O2 led to concentration-dependent DNA strand breakage that was highly-correlated with multiple chemical modifications of all four DNA bases, suggesting that damage is due to hydroxyl radical, OH.. However, the major base damage occurred to adenine. Hence, conclusions made about the occurrence and the extent of oxidative DNA damage on the basis only of changes in 8-hydroxyguanine should be approached with caution.",

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AU - Chimel, Ken

AU - Aruoma, Okezie I.

AU - Cross, Carroll E

AU - Wu, Reen

AU - Halliwell, Barry

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AB - Treatment of human respiratory tract epithelial cells with H2O2 led to concentration-dependent DNA strand breakage that was highly-correlated with multiple chemical modifications of all four DNA bases, suggesting that damage is due to hydroxyl radical, OH.. However, the major base damage occurred to adenine. Hence, conclusions made about the occurrence and the extent of oxidative DNA damage on the basis only of changes in 8-hydroxyguanine should be approached with caution.

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KW - Strand breakage

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