DNA strand breakage and base modification induced by hydrogen peroxide treatment of human respiratory tract epithelial cells

Jeremy P E Spencer, Andrew Jenner, Ken Chimel, Okezie I. Aruoma, Carroll E Cross, Reen Wu, Barry Halliwell

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

Treatment of human respiratory tract epithelial cells with H2O2 led to concentration-dependent DNA strand breakage that was highly-correlated with multiple chemical modifications of all four DNA bases, suggesting that damage is due to hydroxyl radical, OH.. However, the major base damage occurred to adenine. Hence, conclusions made about the occurrence and the extent of oxidative DNA damage on the basis only of changes in 8-hydroxyguanine should be approached with caution.

Original languageEnglish (US)
Pages (from-to)233-236
Number of pages4
JournalFEBS Letters
Volume374
Issue number2
DOIs
StatePublished - Oct 30 1995

Keywords

  • DNA base modification
  • DNA damage
  • GC-MS
  • HO
  • Human respiratory tract epithelial cell
  • Strand breakage

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

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