DNA sequence-specific binding of transformed ah receptor to a dioxin responsive enhancer: Looks aren't everything

M. S. Denison, P. A. Bank, E. F. Yao

Research output: Contribution to journalArticle

Abstract

Gel retardation analysis has been utilized to examine the ability of transformed hepatic cytosolic TCDD:AhR complex to bind to its specific DNA recognition site, the dioxin responsive element (DRE). Although DRE sequence alignment has identified a conserved DNA recognition sequence of C/GNNNC/GTNGCGTGNC/GT/ANNNC/G, site-directed mutagenesis has demonstrated that not all of these bases are important for DNA binding. A putative TCDD:AhR DRE-binding consensus of GCGTGNNA/TNNNG/C has been derived from our studies and it is highly conserved among species. In addition, we present a model for the DNA-binding of transformed TCDD:AhR complex.

Original languageEnglish (US)
Pages (from-to)33-36
Number of pages4
JournalChemosphere
Volume25
Issue number1-2
DOIs
StatePublished - 1992
Externally publishedYes

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry

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