DNA sequence similarity between California isolates of Cryptosporidium parvum

Maria Das Graças C Pereira, Edward R Atwill, Melissa R. Crawford, Rance B Lefebvre

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We evaluated whether nucleic acid amplification with primers specific for Cryptosporidium parvum followed by automated DNA sequence analysis of the PCR amplicons could differentiate between California isolates of C. parvum obtained from livestock, humans, and feral pigs. Almost complete sequence identity existed among the livestock isolates and between the livestock and human isolates. DNA sequences from feral pig isolates differed from those from livestock and humans by 1.0 to 1.2%. The reference sequence obtained by Laxer et al. (M. A. Laxer, B. K. Timblin, and R. J. Patel, Am. J. Trop. Med. Hyg. 45:688-694, 1991.) differed from California isolates of C. parvum by 1.8 to 3.2%. These data suggest that DNA sequence analysis of the amplicon of Laxer et al. does not allow for differentiation between various strains of C. parvum or that our collection of isolates obtained from various hosts from across California was limited to one strain of C. parvum.

Original languageEnglish (US)
Pages (from-to)1584-1586
Number of pages3
JournalApplied and Environmental Microbiology
Volume64
Issue number4
StatePublished - Apr 1998

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Cryptosporidium parvum
Livestock
livestock
nucleotide sequences
DNA
pig
DNA Sequence Analysis
Swine
sequence analysis
nucleic acid
swine
amplification
Nucleic Acids
nucleic acids
Polymerase Chain Reaction
analysis

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

DNA sequence similarity between California isolates of Cryptosporidium parvum. / Pereira, Maria Das Graças C; Atwill, Edward R; Crawford, Melissa R.; Lefebvre, Rance B.

In: Applied and Environmental Microbiology, Vol. 64, No. 4, 04.1998, p. 1584-1586.

Research output: Contribution to journalArticle

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