Abstract
We evaluated whether nucleic acid amplification with primers specific for Cryptosporidium parvum followed by automated DNA sequence analysis of the PCR amplicons could differentiate between California isolates of C. parvum obtained from livestock, humans, and feral pigs. Almost complete sequence identity existed among the livestock isolates and between the livestock and human isolates. DNA sequences from feral pig isolates differed from those from livestock and humans by 1.0 to 1.2%. The reference sequence obtained by Laxer et al. (M. A. Laxer, B. K. Timblin, and R. J. Patel, Am. J. Trop. Med. Hyg. 45:688-694, 1991.) differed from California isolates of C. parvum by 1.8 to 3.2%. These data suggest that DNA sequence analysis of the amplicon of Laxer et al. does not allow for differentiation between various strains of C. parvum or that our collection of isolates obtained from various hosts from across California was limited to one strain of C. parvum.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1584-1586 |
| Number of pages | 3 |
| Journal | Applied and Environmental Microbiology |
| Volume | 64 |
| Issue number | 4 |
| State | Published - Apr 1998 |
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ASJC Scopus subject areas
- Environmental Science(all)
- Biotechnology
- Microbiology
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DNA sequence similarity between California isolates of Cryptosporidium parvum. / Pereira, Maria Das Graças C; Atwill, Edward R; Crawford, Melissa R.; Lefebvre, Rance B.
In: Applied and Environmental Microbiology, Vol. 64, No. 4, 04.1998, p. 1584-1586.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - DNA sequence similarity between California isolates of Cryptosporidium parvum
AU - Pereira, Maria Das Graças C
AU - Atwill, Edward R
AU - Crawford, Melissa R.
AU - Lefebvre, Rance B
PY - 1998/4
Y1 - 1998/4
N2 - We evaluated whether nucleic acid amplification with primers specific for Cryptosporidium parvum followed by automated DNA sequence analysis of the PCR amplicons could differentiate between California isolates of C. parvum obtained from livestock, humans, and feral pigs. Almost complete sequence identity existed among the livestock isolates and between the livestock and human isolates. DNA sequences from feral pig isolates differed from those from livestock and humans by 1.0 to 1.2%. The reference sequence obtained by Laxer et al. (M. A. Laxer, B. K. Timblin, and R. J. Patel, Am. J. Trop. Med. Hyg. 45:688-694, 1991.) differed from California isolates of C. parvum by 1.8 to 3.2%. These data suggest that DNA sequence analysis of the amplicon of Laxer et al. does not allow for differentiation between various strains of C. parvum or that our collection of isolates obtained from various hosts from across California was limited to one strain of C. parvum.
AB - We evaluated whether nucleic acid amplification with primers specific for Cryptosporidium parvum followed by automated DNA sequence analysis of the PCR amplicons could differentiate between California isolates of C. parvum obtained from livestock, humans, and feral pigs. Almost complete sequence identity existed among the livestock isolates and between the livestock and human isolates. DNA sequences from feral pig isolates differed from those from livestock and humans by 1.0 to 1.2%. The reference sequence obtained by Laxer et al. (M. A. Laxer, B. K. Timblin, and R. J. Patel, Am. J. Trop. Med. Hyg. 45:688-694, 1991.) differed from California isolates of C. parvum by 1.8 to 3.2%. These data suggest that DNA sequence analysis of the amplicon of Laxer et al. does not allow for differentiation between various strains of C. parvum or that our collection of isolates obtained from various hosts from across California was limited to one strain of C. parvum.
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M3 - Article
C2 - 9546195
AN - SCOPUS:0031980274
VL - 64
SP - 1584
EP - 1586
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 4
ER -