DNA repair protein Rad55 is a terminal substrate of the DNA damage checkpoints

Vladimir I. Bashkirov, Jeff S. King, Elena V. Bashkirova, Jacqueline Schmuckli-Maurer, Wolf Dietrich Heyer

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Checkpoints, which are integral to the cellular response to DNA damage, coordinate transient cell cycle arrest and the induced expression of DNA repair genes after genotoxic stress. DNA repair ensures cellular survival and genomic stability, utilizing a multipathway network. Here we report evidence that the two systems, DNA damage checkpoint control and DNA repair, are directly connected by demonstrating that the Rad55 double-strand break repair protein of the recombinational repair pathway is a terminal substrate of DNA damage and replication block checkpoints. Rad55p was specifically phosphorylated in response to DNA damage induced by the alkylating agent methyl methanesulfonate, dependent on an active DNA damage checkpoint. Rad55p modification was also observed after gamma ray and UV radiation. The rapid time course of phosphorylation and the recombination defects identified in checkpoint-deficient cells are consistent with a role of the DNA damage checkpoint in activating recombinational repair. Rad55p phosphorylation possibly affects the balance between different competing DNA repair pathways.

Original languageEnglish (US)
Pages (from-to)4393-4404
Number of pages12
JournalMolecular and Cellular Biology
Volume20
Issue number12
DOIs
StatePublished - Jun 2000

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DNA Repair
DNA Damage
Proteins
Phosphorylation
Methyl Methanesulfonate
Genomic Instability
Alkylating Agents
Gamma Rays
Cell Cycle Checkpoints
DNA Replication
Genetic Recombination
Radiation
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Bashkirov, V. I., King, J. S., Bashkirova, E. V., Schmuckli-Maurer, J., & Heyer, W. D. (2000). DNA repair protein Rad55 is a terminal substrate of the DNA damage checkpoints. Molecular and Cellular Biology, 20(12), 4393-4404. https://doi.org/10.1128/MCB.20.12.4393-4404.2000

DNA repair protein Rad55 is a terminal substrate of the DNA damage checkpoints. / Bashkirov, Vladimir I.; King, Jeff S.; Bashkirova, Elena V.; Schmuckli-Maurer, Jacqueline; Heyer, Wolf Dietrich.

In: Molecular and Cellular Biology, Vol. 20, No. 12, 06.2000, p. 4393-4404.

Research output: Contribution to journalArticle

Bashkirov, VI, King, JS, Bashkirova, EV, Schmuckli-Maurer, J & Heyer, WD 2000, 'DNA repair protein Rad55 is a terminal substrate of the DNA damage checkpoints', Molecular and Cellular Biology, vol. 20, no. 12, pp. 4393-4404. https://doi.org/10.1128/MCB.20.12.4393-4404.2000
Bashkirov, Vladimir I. ; King, Jeff S. ; Bashkirova, Elena V. ; Schmuckli-Maurer, Jacqueline ; Heyer, Wolf Dietrich. / DNA repair protein Rad55 is a terminal substrate of the DNA damage checkpoints. In: Molecular and Cellular Biology. 2000 ; Vol. 20, No. 12. pp. 4393-4404.
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