DNA-protein crosslinking in normal human skin fibroblasts exposed to solar ultraviolet wavelengths.

L. W. Lai, Jonathan M Ducore, B. S. Rosenstein

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Three normal human skin fibroblast cell lines were exposed to the simulated solar UV radiation produced by a fluorescent sunlamp under conditions in which the wavelength components shorter than either 295, 305 or 315 nm were excluded. The level of DNA-protein crosslinks (DPC) was then measured in those cells using the alkaline elution technique either immediately after irradiation or following a 24 h incubation. In each case, cells were exposed to fluences that induce similar levels of DPC. For cells exposed to 10 kJ m(-2) of sunlamp UV > 295 nm, the level of DPC exhibited a 2-5-fold increase following incubation. In contrast, 40-100% of the DPC were removed upon incubation of cells irradiated with either 100 kJ m(-2) of sunlamp UV > 305 nm or 150 kJ m(-2) of sunlamp UV > 315 nm. A major difference between the effects induced by these wavelength regions is that, in addition to DPC, a very high level of pyrimidine dimers is also produced by sunlamp UV > 295 nm, whereas much lower dimer yields result from treatment with either sunlamp UV > 305 nm or sunlamp UV > 315 nm. A potential role for type II DNA topoisomerase in the formation of these DPC resulting from either the change in conformational structure caused by the presence of a high level of dimers or an involvement of this enzyme in dimer excision repair is discussed.

Original languageEnglish (US)
Pages (from-to)143-146
Number of pages4
JournalPhotochemistry and Photobiology
Volume46
Issue number1
StatePublished - Jul 1987
Externally publishedYes

Fingerprint

fibroblasts
crosslinking
Fibroblasts
Crosslinking
Skin
deoxyribonucleic acid
proteins
Wavelength
DNA
wavelengths
Dimers
dimers
Proteins
Cells
Pyrimidine Dimers
cells
Type II DNA Topoisomerase
elution
Solar radiation
DNA Repair

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

DNA-protein crosslinking in normal human skin fibroblasts exposed to solar ultraviolet wavelengths. / Lai, L. W.; Ducore, Jonathan M; Rosenstein, B. S.

In: Photochemistry and Photobiology, Vol. 46, No. 1, 07.1987, p. 143-146.

Research output: Contribution to journalArticle

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