DNA-protein crosslinking in normal and solar UV-sensitive ICR 2A frog cell lines exposed to solar UV-radiation

Barry S. Rosenstein, Li Wen Lai, Jonathan M Ducore, Rebecca B. Rosenstein

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

DNA-protein crosslinks (DPC) were measured following exposure to the solar UV wavelengths produced by a fluorescent sunlamp in ICR 2A frog cells and two solar UV-sensitive mutants derived from this cell line. Approx. 5-7 DPC per 1010 dalton were induced in these cells by either 150 kJ/m2 of sunlamp UV > 315 nm plus photoreactivating light (PRL) or 10 kJ/m2 of sunlamp UV > 295 nm. The irradiated cells were then incubated for 0-24 h and the level of DPC measured using alkaline elution. It was found for the ICR 2A cells exposed to sunlamp UV > 315 nm that the level of DPC increased about 3-fold during a 2-h postirradiation incubation and then decreased. The mutant cell lines also showed an enhancement in the level of DPC following irradiation, although it was much less pronounced and the levels decreased much more rapidly. In a similar fashion, the level of DPC increased in ICR 2A cells exposed to sunlamp UV > 295 nm with more than a 5-fold enhancement after a 4-h incubation. Once again, the mutant cell lines showed an increase in the level of DPC that was smaller and more transient than the effect in the ICR 2A cells. These results suggests that this enhancement in DPC may be indicative of a process that plays a role in cellular survival following solar UV-irradiation.

Original languageEnglish (US)
Pages (from-to)219-226
Number of pages8
JournalMutation Research-DNA Repair
Volume217
Issue number3
DOIs
StatePublished - 1989
Externally publishedYes

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Keywords

  • Alkaline elution
  • DNA-protein crosslinks
  • ICR 2A cells
  • Solar UV-radiation

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Toxicology
  • Medicine(all)

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