DNA packaging steps in bacteriophage lambda head assembly

Dale Kaiser, Michael Syvanen, Terrie Masuda

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Petit λ is an empty spherical shell of protein which appears wherever λ grows. If phage DNA and petit λ are added to a cell-free extract of induced lysogenic bacteria, then phage particles are formed that contain the DNA and protein from the petit λ. Petit λ is transformed, without dissociation, into a phage head by addition of DNA and more phage proteins. The products of ten genes, nine phage and one host, are required for λ head assembly. Among these, the products of four phage genes, E, B, C, and Nu3 and of the host gene groE are involved in the synthesis of petit λ, consequently these proteins are dispensable for head assembly in extracts to which petit λ has been added. The products of genes A and D allow DNA to combine with petit λ to form a head that has normal morphology. In an extract, DNA can react with A product and petit λ to become partially DNAase-resistant, as if an unstable DNA-filled intermediate were formed. ATP and spermidine are needed at this stage. This intermediate is subsequently stabilized by addition of D product. The data suggest a pathway for head assembly.

Original languageEnglish (US)
JournalJournal of Molecular Biology
Volume91
Issue number2
DOIs
StatePublished - Jan 15 1975
Externally publishedYes

Fingerprint

DNA Packaging
Bacteriophage lambda
Bacteriophages
DNA
Head
Genes
Proteins
Spermidine
Deoxyribonucleases
Cell Extracts
Adenosine Triphosphate
Bacteria

ASJC Scopus subject areas

  • Virology

Cite this

DNA packaging steps in bacteriophage lambda head assembly. / Kaiser, Dale; Syvanen, Michael; Masuda, Terrie.

In: Journal of Molecular Biology, Vol. 91, No. 2, 15.01.1975.

Research output: Contribution to journalArticle

@article{06ebe26a40b64362a6afb07212596ccf,
title = "DNA packaging steps in bacteriophage lambda head assembly",
abstract = "Petit λ is an empty spherical shell of protein which appears wherever λ grows. If phage DNA and petit λ are added to a cell-free extract of induced lysogenic bacteria, then phage particles are formed that contain the DNA and protein from the petit λ. Petit λ is transformed, without dissociation, into a phage head by addition of DNA and more phage proteins. The products of ten genes, nine phage and one host, are required for λ head assembly. Among these, the products of four phage genes, E, B, C, and Nu3 and of the host gene groE are involved in the synthesis of petit λ, consequently these proteins are dispensable for head assembly in extracts to which petit λ has been added. The products of genes A and D allow DNA to combine with petit λ to form a head that has normal morphology. In an extract, DNA can react with A product and petit λ to become partially DNAase-resistant, as if an unstable DNA-filled intermediate were formed. ATP and spermidine are needed at this stage. This intermediate is subsequently stabilized by addition of D product. The data suggest a pathway for head assembly.",
author = "Dale Kaiser and Michael Syvanen and Terrie Masuda",
year = "1975",
month = "1",
day = "15",
doi = "10.1016/0022-2836(75)90158-8",
language = "English (US)",
volume = "91",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - DNA packaging steps in bacteriophage lambda head assembly

AU - Kaiser, Dale

AU - Syvanen, Michael

AU - Masuda, Terrie

PY - 1975/1/15

Y1 - 1975/1/15

N2 - Petit λ is an empty spherical shell of protein which appears wherever λ grows. If phage DNA and petit λ are added to a cell-free extract of induced lysogenic bacteria, then phage particles are formed that contain the DNA and protein from the petit λ. Petit λ is transformed, without dissociation, into a phage head by addition of DNA and more phage proteins. The products of ten genes, nine phage and one host, are required for λ head assembly. Among these, the products of four phage genes, E, B, C, and Nu3 and of the host gene groE are involved in the synthesis of petit λ, consequently these proteins are dispensable for head assembly in extracts to which petit λ has been added. The products of genes A and D allow DNA to combine with petit λ to form a head that has normal morphology. In an extract, DNA can react with A product and petit λ to become partially DNAase-resistant, as if an unstable DNA-filled intermediate were formed. ATP and spermidine are needed at this stage. This intermediate is subsequently stabilized by addition of D product. The data suggest a pathway for head assembly.

AB - Petit λ is an empty spherical shell of protein which appears wherever λ grows. If phage DNA and petit λ are added to a cell-free extract of induced lysogenic bacteria, then phage particles are formed that contain the DNA and protein from the petit λ. Petit λ is transformed, without dissociation, into a phage head by addition of DNA and more phage proteins. The products of ten genes, nine phage and one host, are required for λ head assembly. Among these, the products of four phage genes, E, B, C, and Nu3 and of the host gene groE are involved in the synthesis of petit λ, consequently these proteins are dispensable for head assembly in extracts to which petit λ has been added. The products of genes A and D allow DNA to combine with petit λ to form a head that has normal morphology. In an extract, DNA can react with A product and petit λ to become partially DNAase-resistant, as if an unstable DNA-filled intermediate were formed. ATP and spermidine are needed at this stage. This intermediate is subsequently stabilized by addition of D product. The data suggest a pathway for head assembly.

UR - http://www.scopus.com/inward/record.url?scp=0016440137&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016440137&partnerID=8YFLogxK

U2 - 10.1016/0022-2836(75)90158-8

DO - 10.1016/0022-2836(75)90158-8

M3 - Article

C2 - 1102700

AN - SCOPUS:0016440137

VL - 91

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 2

ER -