DNA microarray experiments: Biological and technological aspects

Danh V. Nguyen, A. Bulak Arpat, Naisyin Wang, Raymond J. Carroll

Research output: Contribution to journalArticle

123 Scopus citations

Abstract

DNA microarray technologies, such as cDNA and oligonucleotide microarrays, promise to revolutionize biological research and further our understanding of biological processes. Due to the complex nature and sheer amount of data produced from microarray experiments, biologists have sought the collaboration of experts in the analytical sciences, including statisticians, among others. However, the biological and technical intricacies of microarray experiments are not easily accessible to analytical experts. One aim for this review is to provide a bridge to some of the relevant biological and technical aspects involved in microarray experiments. While there is already a large literature on the broad applications of the technology, basic research on the technology itself and studies to understand process variation remain in their infancy. We emphasize the importance of basic research in DNA array technologies to improve the reliability of future experiments.

Original languageEnglish (US)
Pages (from-to)701-717
Number of pages17
JournalBiometrics
Volume58
Issue number4
StatePublished - Dec 2002
Externally publishedYes

Keywords

  • Affymetrix
  • cDNA
  • Design of experiments
  • Gene expression
  • Image processing
  • Microarray
  • Molecular biology
  • Normalization
  • Nucleotide labeling
  • Oligonucleotide
  • Reverse transcription
  • Transcription
  • Variability

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Public Health, Environmental and Occupational Health
  • Agricultural and Biological Sciences (miscellaneous)
  • Applied Mathematics
  • Statistics and Probability

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  • Cite this

    Nguyen, D. V., Arpat, A. B., Wang, N., & Carroll, R. J. (2002). DNA microarray experiments: Biological and technological aspects. Biometrics, 58(4), 701-717.