DNA microarray experiments: Biological and technological aspects

Danh V. Nguyen, A. Bulak Arpat, Naisyin Wang, Raymond J. Carroll

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

DNA microarray technologies, such as cDNA and oligonucleotide microarrays, promise to revolutionize biological research and further our understanding of biological processes. Due to the complex nature and sheer amount of data produced from microarray experiments, biologists have sought the collaboration of experts in the analytical sciences, including statisticians, among others. However, the biological and technical intricacies of microarray experiments are not easily accessible to analytical experts. One aim for this review is to provide a bridge to some of the relevant biological and technical aspects involved in microarray experiments. While there is already a large literature on the broad applications of the technology, basic research on the technology itself and studies to understand process variation remain in their infancy. We emphasize the importance of basic research in DNA array technologies to improve the reliability of future experiments.

Original languageEnglish (US)
Pages (from-to)701-717
Number of pages17
JournalBiometrics
Volume58
Issue number4
StatePublished - Dec 2002
Externally publishedYes

Fingerprint

DNA Microarray
Microarrays
Oligonucleotide Array Sequence Analysis
Microarray
DNA
Technology
Experiment
microarray technology
application technology
Experiments
Research
infancy
oligonucleotides
Biological Phenomena
Process Variation
biologists
CDNA
Oligonucleotides
Complementary DNA
DNA microarrays

Keywords

  • Affymetrix
  • cDNA
  • Design of experiments
  • Gene expression
  • Image processing
  • Microarray
  • Molecular biology
  • Normalization
  • Nucleotide labeling
  • Oligonucleotide
  • Reverse transcription
  • Transcription
  • Variability

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Public Health, Environmental and Occupational Health
  • Agricultural and Biological Sciences (miscellaneous)
  • Applied Mathematics
  • Statistics and Probability

Cite this

Nguyen, D. V., Arpat, A. B., Wang, N., & Carroll, R. J. (2002). DNA microarray experiments: Biological and technological aspects. Biometrics, 58(4), 701-717.

DNA microarray experiments : Biological and technological aspects. / Nguyen, Danh V.; Arpat, A. Bulak; Wang, Naisyin; Carroll, Raymond J.

In: Biometrics, Vol. 58, No. 4, 12.2002, p. 701-717.

Research output: Contribution to journalArticle

Nguyen, DV, Arpat, AB, Wang, N & Carroll, RJ 2002, 'DNA microarray experiments: Biological and technological aspects', Biometrics, vol. 58, no. 4, pp. 701-717.
Nguyen DV, Arpat AB, Wang N, Carroll RJ. DNA microarray experiments: Biological and technological aspects. Biometrics. 2002 Dec;58(4):701-717.
Nguyen, Danh V. ; Arpat, A. Bulak ; Wang, Naisyin ; Carroll, Raymond J. / DNA microarray experiments : Biological and technological aspects. In: Biometrics. 2002 ; Vol. 58, No. 4. pp. 701-717.
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