DNA ligase and the pyridine nucleotide cycle in Salmonella typhimurium

U. E. Park, B. M. Olivera, K. T. Hughes, J. R. Roth, D. R. Hillyard

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Bacterial DNA ligases use NAD as an energy source. In this study we addressed two questions about these enzymes. First, what is the physiological consequence of completely removing the NAD-dependent enzyme and replacing it with an ATP-dependent DNA ligase? We constructed Salmonella typhimurium strains in which the endogenous NAD-dependent DNA ligase activity was inactivated by an insertion mutation and the ATP-dependent enzyme from bacteriophage T4 was provided by a cloned phage gene. Such strains were physiologically indistinguishable from the wild type, even under conditions of UV irradiation or treatment with alkylating agents. These results suggest that specific functional interactions between DNA ligase and other replication and repair enzymes may be unimportant under the conditions tested. Second, the importance of DNA ilgation as the initiating event of the bacterial pyridine nucleotide cycle was critically assessed in these mutant strains. Surprisingly, our results indicate that DNA ligation makes a minimal contribution to the pyridine nucleotide cycle; the Salmonella strains with only an ATP-dependent ligase had the same NAD turnover rates as the wild-type strain with an NAD-dependent ligase. However, we found that NAD turnover was significantly decreased under anaerobic conditions. We suggest that most intracellular pyridine nucleotide breakdown occurs in a process that protects the cell against oxygen damage but involves a biochemical mechanism other than DNA ligation.

Original languageEnglish (US)
Pages (from-to)2173-2180
Number of pages8
JournalJournal of Bacteriology
Volume171
Issue number4
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Fingerprint Dive into the research topics of 'DNA ligase and the pyridine nucleotide cycle in Salmonella typhimurium'. Together they form a unique fingerprint.

  • Cite this

    Park, U. E., Olivera, B. M., Hughes, K. T., Roth, J. R., & Hillyard, D. R. (1989). DNA ligase and the pyridine nucleotide cycle in Salmonella typhimurium. Journal of Bacteriology, 171(4), 2173-2180.