DNA immunization against respiratory syncytial virus (RSV) in infant rhesus monkeys

Kerrie Vaughan, Gary H. Rhodes, Laurel J Gershwin

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

A DNA vaccine was tested in infant Rhesus macaques to evaluate its safety, immunogenicity and protective efficacy. Monkeys were vaccinated and challenged with a clinical isolate of human RSV. Vaccinated animals developed humoral and cellular responses following inoculation with plasmid DNA encoding the fusion (F) and nucleoprotein (N), from closely related bovine RSV. Vaccinated monkeys had decreased RSV in their lungs post-infection, and there was a qualitative difference in histopathology observed between vaccinated and unvaccinated animals. The combined result of safety and immunogenicity in a neonatal primate model is encouraging, suggesting the feasibility of DNA vaccines against RSV in infants.

Original languageEnglish (US)
Pages (from-to)2928-2942
Number of pages15
JournalVaccine
Volume23
Issue number22
DOIs
StatePublished - Apr 22 2005

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Keywords

  • DNA immunization
  • Rhesus monkeys
  • RSV

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

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