Abstract
Treatment of human respiratory tract tracheobronchial epithelial cells with gas-phase cigarette smoke led to dose-dependent DNA strand breakage that was highly correlated with multiple chemical modifications of all four DNA bases. The pattern of base damage suggests attack by hydroxyl radicals (OH:). However, by far the most important base damage in quantitative terms was formation of xanthine and hypoxanthine, presumably resulting from deamination of guanine and adenine respectively. Hence, DNA damage by cigarette smoke may involve reactive nitrogen species as well as reactive oxygen species.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 179-182 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 375 |
| Issue number | 3 |
| DOIs | |
| State | Published - Nov 20 1995 |
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Keywords
- Cigarette smoke
- DNA base modification
- DNA damage
- GC-MS
- Human respiratory tract cell
- Strand breakage
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Genetics
- Molecular Biology
- Structural Biology
Cite this
DNA damage in human respiratory tract epithelial cells : damage by gas phase cigarette smoke apparently involves attack by reactive nitrogen species in addition to oxygen radicals. / Spencer, Jeremy P E; Jenner, Andrew; Chimel, Ken; Aruoma, Okezie I.; Cross, Carroll E; Wu, Reen; Halliwell, Barry.
In: FEBS Letters, Vol. 375, No. 3, 20.11.1995, p. 179-182.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - DNA damage in human respiratory tract epithelial cells
T2 - damage by gas phase cigarette smoke apparently involves attack by reactive nitrogen species in addition to oxygen radicals
AU - Spencer, Jeremy P E
AU - Jenner, Andrew
AU - Chimel, Ken
AU - Aruoma, Okezie I.
AU - Cross, Carroll E
AU - Wu, Reen
AU - Halliwell, Barry
PY - 1995/11/20
Y1 - 1995/11/20
N2 - Treatment of human respiratory tract tracheobronchial epithelial cells with gas-phase cigarette smoke led to dose-dependent DNA strand breakage that was highly correlated with multiple chemical modifications of all four DNA bases. The pattern of base damage suggests attack by hydroxyl radicals (OH:). However, by far the most important base damage in quantitative terms was formation of xanthine and hypoxanthine, presumably resulting from deamination of guanine and adenine respectively. Hence, DNA damage by cigarette smoke may involve reactive nitrogen species as well as reactive oxygen species.
AB - Treatment of human respiratory tract tracheobronchial epithelial cells with gas-phase cigarette smoke led to dose-dependent DNA strand breakage that was highly correlated with multiple chemical modifications of all four DNA bases. The pattern of base damage suggests attack by hydroxyl radicals (OH:). However, by far the most important base damage in quantitative terms was formation of xanthine and hypoxanthine, presumably resulting from deamination of guanine and adenine respectively. Hence, DNA damage by cigarette smoke may involve reactive nitrogen species as well as reactive oxygen species.
KW - Cigarette smoke
KW - DNA base modification
KW - DNA damage
KW - GC-MS
KW - Human respiratory tract cell
KW - Strand breakage
UR - http://www.scopus.com/inward/record.url?scp=0028788798&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028788798&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(95)01199-O
DO - 10.1016/0014-5793(95)01199-O
M3 - Article
VL - 375
SP - 179
EP - 182
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -