DNA damage in human respiratory tract epithelial cells: damage by gas phase cigarette smoke apparently involves attack by reactive nitrogen species in addition to oxygen radicals

Jeremy P E Spencer, Andrew Jenner, Ken Chimel, Okezie I. Aruoma, Carroll E Cross, Reen Wu, Barry Halliwell

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

Treatment of human respiratory tract tracheobronchial epithelial cells with gas-phase cigarette smoke led to dose-dependent DNA strand breakage that was highly correlated with multiple chemical modifications of all four DNA bases. The pattern of base damage suggests attack by hydroxyl radicals (OH:). However, by far the most important base damage in quantitative terms was formation of xanthine and hypoxanthine, presumably resulting from deamination of guanine and adenine respectively. Hence, DNA damage by cigarette smoke may involve reactive nitrogen species as well as reactive oxygen species.

Original languageEnglish (US)
Pages (from-to)179-182
Number of pages4
JournalFEBS Letters
Volume375
Issue number3
DOIs
StatePublished - Nov 20 1995

Keywords

  • Cigarette smoke
  • DNA base modification
  • DNA damage
  • GC-MS
  • Human respiratory tract cell
  • Strand breakage

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

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