DNA crosslinking and cytotoxicity in normal and transformed human cells treated with antitumor nitrosoureas

L. C. Erickson, M. O. Bradley, Jonathan M Ducore, R. A. Ewig, K. W. Kohn

Research output: Contribution to journalArticle

179 Citations (Scopus)

Abstract

Normal (IMR-90) and simian virus 40-transformed (VA-13) human embryo cells were treated with antitumor nitrosoureas, and the effects on cell viability and cell DNA were compared. All six nitrosoureas tested were more toxic to VA-13 cells than to IMR-90 cells as measured by decrease in cell proliferation or in colony formation. The nitrosoureas capable of generating alkylisocyanates produced a smaller difference between the cell types than did derivatives lacking this capacity. DNA damage was measured by alkaline elution in cells treated with four chloroethylnitrosoureas. Whereas VA-13 cells exhibited dose-dependent interstrand crosslinking, little or none was detected in IMR-90 cells. The IMR-90 cells, however, exhibited at least as much DNA-protein crosslinking as did VA-13 cells. The results can be interpreted in terms of a possible difference in DNA repair between the cell lines.

Original languageEnglish (US)
Pages (from-to)467-471
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume77
Issue number1
StatePublished - 1980
Externally publishedYes

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DNA
Simian virus 40
Poisons
DNA Repair
DNA Damage
Cell Survival
Embryonic Structures
Cell Proliferation
Cell Line
Proteins

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

DNA crosslinking and cytotoxicity in normal and transformed human cells treated with antitumor nitrosoureas. / Erickson, L. C.; Bradley, M. O.; Ducore, Jonathan M; Ewig, R. A.; Kohn, K. W.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 77, No. 1, 1980, p. 467-471.

Research output: Contribution to journalArticle

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