DNA array analyses of Arabidopsis thaliana lacking a vacuolar Na +/H+ antiporter: Impact of AtNHX1 on gene expression

Jordan B. Sottosanto, Angela C Gelli, Eduardo Blumwald

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

AtNHX1, a vacuolar cation/proton antiporter of Arabidopsis, plays an important role in salt tolerance, ion homeostasis and development. We used the T-DNA insertional mutant of AtNHX1 (nhx1 plants) and Affymetrix ATH1 DNA arrays to assess differences in transcriptional profiles and further characterize the roles of a vacuolar cation/proton antiporter. Mature, soil-grown plants were used in this study to approximate typical physiological growing conditions. A comparison of plants grown in the absence of salt stress yielded many transcripts that were affected by the absence of the AtNHX1 vacuolar antiporter. Furthermore, changes in gene expression dueto a non-lethal salt stress (100 mM NaCl) in the nhx1 plants were significantly different from the changes seen in wild-type plants. The nhx1 transcriptome was differentially affected when the plants were grown in the absence or presence of salt. In conclusion, in addition to the known role(s) of AtNHX1 on ion homeostasis, the vacuolar cation/proton antiporter plays a significant role in intracellular vesicular trafficking, protein targeting, and other cellular processes.

Original languageEnglish (US)
Pages (from-to)752-771
Number of pages20
JournalPlant Journal
Volume40
Issue number5
DOIs
StatePublished - Dec 2004

Fingerprint

Sodium-Hydrogen Antiporter
Oligonucleotide Array Sequence Analysis
Arabidopsis
Antiporters
antiporters
Arabidopsis thaliana
Gene Expression
gene expression
DNA
Cations
Protons
Salts
salt stress
homeostasis
Homeostasis
Ions
ions
Salt-Tolerance
protein transport
Protein Transport

Keywords

  • AtNHX1
  • DNA arrays
  • Ion homeostasis
  • Knockout
  • Vacuolar Na/H antiporter
  • Vesicular trafficking

ASJC Scopus subject areas

  • Plant Science

Cite this

DNA array analyses of Arabidopsis thaliana lacking a vacuolar Na +/H+ antiporter : Impact of AtNHX1 on gene expression. / Sottosanto, Jordan B.; Gelli, Angela C; Blumwald, Eduardo.

In: Plant Journal, Vol. 40, No. 5, 12.2004, p. 752-771.

Research output: Contribution to journalArticle

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