Abstract
AtNHX1, a vacuolar cation/proton antiporter of Arabidopsis, plays an important role in salt tolerance, ion homeostasis and development. We used the T-DNA insertional mutant of AtNHX1 (nhx1 plants) and Affymetrix ATH1 DNA arrays to assess differences in transcriptional profiles and further characterize the roles of a vacuolar cation/proton antiporter. Mature, soil-grown plants were used in this study to approximate typical physiological growing conditions. A comparison of plants grown in the absence of salt stress yielded many transcripts that were affected by the absence of the AtNHX1 vacuolar antiporter. Furthermore, changes in gene expression dueto a non-lethal salt stress (100 mM NaCl) in the nhx1 plants were significantly different from the changes seen in wild-type plants. The nhx1 transcriptome was differentially affected when the plants were grown in the absence or presence of salt. In conclusion, in addition to the known role(s) of AtNHX1 on ion homeostasis, the vacuolar cation/proton antiporter plays a significant role in intracellular vesicular trafficking, protein targeting, and other cellular processes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 752-771 |
| Number of pages | 20 |
| Journal | Plant Journal |
| Volume | 40 |
| Issue number | 5 |
| DOIs | |
| State | Published - Dec 2004 |
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Keywords
- AtNHX1
- DNA arrays
- Ion homeostasis
- Knockout
- Vacuolar Na/H antiporter
- Vesicular trafficking
ASJC Scopus subject areas
- Plant Science
Cite this
DNA array analyses of Arabidopsis thaliana lacking a vacuolar Na +/H+ antiporter : Impact of AtNHX1 on gene expression. / Sottosanto, Jordan B.; Gelli, Angela C; Blumwald, Eduardo.
In: Plant Journal, Vol. 40, No. 5, 12.2004, p. 752-771.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - DNA array analyses of Arabidopsis thaliana lacking a vacuolar Na +/H+ antiporter
T2 - Impact of AtNHX1 on gene expression
AU - Sottosanto, Jordan B.
AU - Gelli, Angela C
AU - Blumwald, Eduardo
PY - 2004/12
Y1 - 2004/12
N2 - AtNHX1, a vacuolar cation/proton antiporter of Arabidopsis, plays an important role in salt tolerance, ion homeostasis and development. We used the T-DNA insertional mutant of AtNHX1 (nhx1 plants) and Affymetrix ATH1 DNA arrays to assess differences in transcriptional profiles and further characterize the roles of a vacuolar cation/proton antiporter. Mature, soil-grown plants were used in this study to approximate typical physiological growing conditions. A comparison of plants grown in the absence of salt stress yielded many transcripts that were affected by the absence of the AtNHX1 vacuolar antiporter. Furthermore, changes in gene expression dueto a non-lethal salt stress (100 mM NaCl) in the nhx1 plants were significantly different from the changes seen in wild-type plants. The nhx1 transcriptome was differentially affected when the plants were grown in the absence or presence of salt. In conclusion, in addition to the known role(s) of AtNHX1 on ion homeostasis, the vacuolar cation/proton antiporter plays a significant role in intracellular vesicular trafficking, protein targeting, and other cellular processes.
AB - AtNHX1, a vacuolar cation/proton antiporter of Arabidopsis, plays an important role in salt tolerance, ion homeostasis and development. We used the T-DNA insertional mutant of AtNHX1 (nhx1 plants) and Affymetrix ATH1 DNA arrays to assess differences in transcriptional profiles and further characterize the roles of a vacuolar cation/proton antiporter. Mature, soil-grown plants were used in this study to approximate typical physiological growing conditions. A comparison of plants grown in the absence of salt stress yielded many transcripts that were affected by the absence of the AtNHX1 vacuolar antiporter. Furthermore, changes in gene expression dueto a non-lethal salt stress (100 mM NaCl) in the nhx1 plants were significantly different from the changes seen in wild-type plants. The nhx1 transcriptome was differentially affected when the plants were grown in the absence or presence of salt. In conclusion, in addition to the known role(s) of AtNHX1 on ion homeostasis, the vacuolar cation/proton antiporter plays a significant role in intracellular vesicular trafficking, protein targeting, and other cellular processes.
KW - AtNHX1
KW - DNA arrays
KW - Ion homeostasis
KW - Knockout
KW - Vacuolar Na/H antiporter
KW - Vesicular trafficking
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UR - http://www.scopus.com/inward/citedby.url?scp=10344237576&partnerID=8YFLogxK
U2 - 10.1111/j.1365-313X.2004.02253.x
DO - 10.1111/j.1365-313X.2004.02253.x
M3 - Article
C2 - 15546358
AN - SCOPUS:10344237576
VL - 40
SP - 752
EP - 771
JO - Plant Journal
JF - Plant Journal
SN - 0960-7412
IS - 5
ER -