DNA and protein analyses of tick-derived isolates of Borrelia burgdorferi from California

Rance B Lefebvre, R. S. Lane, G. C. Perng, J. A. Brown, R. C. Johnson

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Nine isolates of Borrelia burgdorferi from ixodid ticks collected in northern California were characterized. Restriction endonuclease analysis, pulsed-field gel electrophoresis, and Western blot (immunoblot) analysis were used in this study. Four isolates were very similar to each other. The others shared some similarities but were classified as having unique genotypes. A strain from an Ixodes neotomae tick displayed the greatest genetic and antigenic diversity when compared to the isolates collected from Ixodes pacificus ticks. A computerized library based on DNA banding patterns of the isolates by restriction enzyme analysis is also reported. This library was created by using a scanning laser densitometer.

Original languageEnglish (US)
Pages (from-to)700-707
Number of pages8
JournalJournal of Clinical Microbiology
Volume28
Issue number4
StatePublished - 1990

Fingerprint

Arthropod Proteins
Borrelia burgdorferi
Ticks
Ixodes
Libraries
DNA
Western Blotting
Antigenic Variation
Restriction Mapping
Pulsed Field Gel Electrophoresis
DNA Restriction Enzymes
Lasers
Genotype

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

DNA and protein analyses of tick-derived isolates of Borrelia burgdorferi from California. / Lefebvre, Rance B; Lane, R. S.; Perng, G. C.; Brown, J. A.; Johnson, R. C.

In: Journal of Clinical Microbiology, Vol. 28, No. 4, 1990, p. 700-707.

Research output: Contribution to journalArticle

Lefebvre, Rance B ; Lane, R. S. ; Perng, G. C. ; Brown, J. A. ; Johnson, R. C. / DNA and protein analyses of tick-derived isolates of Borrelia burgdorferi from California. In: Journal of Clinical Microbiology. 1990 ; Vol. 28, No. 4. pp. 700-707.
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