Diversity of pulsed-field gel electrophoresis pulsotypes, serovars, and antibiotic resistance among salmonella isolates from wild amphibians and reptiles in the California Central Coast

Lisa Gorski, Michele T Jay-Russell, Anita S. Liang, Samarpita Walker, Yingjia Bengson, Jessica Govoni, Robert E. Mandrell

Research output: Contribution to journalReview article

21 Citations (Scopus)

Abstract

A survey of cold-blooded vertebrates and associated surface waters in a produce-growing region on the Central California Coast was done between May and September 2011 to determine the diversity of Salmonella. Samples from 460 amphibians and reptiles and 119 water samples were collected and cultured for Salmonella. Animals sampled were frogs (n=331), lizards (n=59), newts (n=5), salamanders (n=6), snakes (n=39), and toads (n=20). Salmonella was isolated from 37 individual animals, including frogs, lizards, snakes, and toads. Snakes were the most likely to contain Salmonella, with 59% testing positive followed by 15.3% of lizards, 5% of toads, and 1.2% of frogs. Fifteen water samples (12.6%) were positive. Twenty-two different serovars were identified, and the majority of isolates were S. enterica subsp. IIIb, with subsp. I, II, and IIIa also found. The serovar isolated most frequently was S. enterica subsp. IIIb 16:z10:e,n,x,z15, from snakes and frogs in five different locations. S. enterica subsp. I serovar Typhimurium and the monophasic I 6,8:d:- were isolated from water, and subspecies I Duisburg and its variants were found in animals and water. Some samples contained more than one type of Salmonella. Analysis of pulsed-field gel electrophoresis pulsotypes indicated that some strains persisted in animals and water collected from the same location. Sixty-six isolates displayed antibiotic resistance, with 27 isolates resistant to more than one antibiotic, including a subspecies IIIb isolate from snake having resistance to five different antibiotics. Twenty-three isolates were resistant to more than one class of antibiotic, and six isolates were resistant to three classes. While these subspecies of IIIa and IIIb cause fewer instances of human illness, they may serve as reservoirs of antibiotic resistance, determinants in the environment, and be sources of contamination of leafy greens associated with product recalls.

Original languageEnglish (US)
Pages (from-to)540-548
Number of pages9
JournalFoodborne Pathogens and Disease
Volume10
Issue number6
DOIs
StatePublished - Jun 1 2013

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Reptiles
Pulsed Field Gel Electrophoresis
Amphibians
pulsed-field gel electrophoresis
Microbial Drug Resistance
Snakes
Salmonella
antibiotic resistance
Anura
snakes
reptiles
amphibians
serotypes
frogs
coasts
toads
Lizards
Water
lizards
antibiotics

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Food Science
  • Animal Science and Zoology

Cite this

Diversity of pulsed-field gel electrophoresis pulsotypes, serovars, and antibiotic resistance among salmonella isolates from wild amphibians and reptiles in the California Central Coast. / Gorski, Lisa; Jay-Russell, Michele T; Liang, Anita S.; Walker, Samarpita; Bengson, Yingjia; Govoni, Jessica; Mandrell, Robert E.

In: Foodborne Pathogens and Disease, Vol. 10, No. 6, 01.06.2013, p. 540-548.

Research output: Contribution to journalReview article

Gorski, Lisa ; Jay-Russell, Michele T ; Liang, Anita S. ; Walker, Samarpita ; Bengson, Yingjia ; Govoni, Jessica ; Mandrell, Robert E. / Diversity of pulsed-field gel electrophoresis pulsotypes, serovars, and antibiotic resistance among salmonella isolates from wild amphibians and reptiles in the California Central Coast. In: Foodborne Pathogens and Disease. 2013 ; Vol. 10, No. 6. pp. 540-548.
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abstract = "A survey of cold-blooded vertebrates and associated surface waters in a produce-growing region on the Central California Coast was done between May and September 2011 to determine the diversity of Salmonella. Samples from 460 amphibians and reptiles and 119 water samples were collected and cultured for Salmonella. Animals sampled were frogs (n=331), lizards (n=59), newts (n=5), salamanders (n=6), snakes (n=39), and toads (n=20). Salmonella was isolated from 37 individual animals, including frogs, lizards, snakes, and toads. Snakes were the most likely to contain Salmonella, with 59{\%} testing positive followed by 15.3{\%} of lizards, 5{\%} of toads, and 1.2{\%} of frogs. Fifteen water samples (12.6{\%}) were positive. Twenty-two different serovars were identified, and the majority of isolates were S. enterica subsp. IIIb, with subsp. I, II, and IIIa also found. The serovar isolated most frequently was S. enterica subsp. IIIb 16:z10:e,n,x,z15, from snakes and frogs in five different locations. S. enterica subsp. I serovar Typhimurium and the monophasic I 6,8:d:- were isolated from water, and subspecies I Duisburg and its variants were found in animals and water. Some samples contained more than one type of Salmonella. Analysis of pulsed-field gel electrophoresis pulsotypes indicated that some strains persisted in animals and water collected from the same location. Sixty-six isolates displayed antibiotic resistance, with 27 isolates resistant to more than one antibiotic, including a subspecies IIIb isolate from snake having resistance to five different antibiotics. Twenty-three isolates were resistant to more than one class of antibiotic, and six isolates were resistant to three classes. While these subspecies of IIIa and IIIb cause fewer instances of human illness, they may serve as reservoirs of antibiotic resistance, determinants in the environment, and be sources of contamination of leafy greens associated with product recalls.",
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