Divergent regulation of ryanodine receptor 2 calcium release channels by arrhythmogenic human calmodulin missense mutants

Hyun Seok Hwang, Florentin R. Nitu, Yi Yang, Kafa Walweel, Laetitia Pereira, Christopher N. Johnson, Michela Faggioni, Walter J. Chazin, Derek Laver, Alfred L. George, Razvan L. Cornea, Donald M Bers, Björn C. Knollmann

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

RATIONALE:: Calmodulin (CaM) mutations are associated with an autosomal dominant syndrome of ventricular arrhythmia and sudden death that can present with divergent clinical features of catecholaminergic polymorphic ventricular tachycardia (CPVT) or long QT syndrome (LQTS). CaM binds to and inhibits ryanodine receptor (RyR2) Ca release channels in the heart, but whether arrhythmogenic CaM mutants alter RyR2 function is not known. OBJECTIVE:: To gain mechanistic insight into how human CaM mutations affect RyR2 Ca channels. METHODS AND RESULTS:: We studied recombinant CaM mutants associated with CPVT (N54I and N98S) or LQTS (D96V, D130G, and F142L). As a group, all LQTS-associated CaM mutants (LQTS-CaMs) exhibited reduced Ca affinity, whereas CPVT-associated CaM mutants (CPVT-CaMs) had either normal or modestly lower Ca affinity. In permeabilized ventricular myocytes, CPVT-CaMs at a physiological intracellular concentration (100 nmol/L) promoted significantly higher spontaneous Ca wave and spark activity, a typical cellular phenotype of CPVT. Compared with wild-type CaM, CPVT-CaMs caused greater RyR2 single-channel open probability and showed enhanced binding affinity to RyR2. Even a 1:8 mixture of CPVT-CaM:wild-type-CaM activated Ca waves, demonstrating functional dominance. In contrast, LQTS-CaMs did not promote Ca waves and exhibited either normal regulation of RyR2 single channels (D96V) or lower RyR2-binding affinity (D130G and F142L). None of the CaM mutants altered Ca/CaM binding to CaM-kinase II. CONCLUSIONS:: A small proportion of CPVT-CaM is sufficient to evoke arrhythmogenic Ca disturbances, whereas LQTS-CaMs do not. Our findings explain the clinical presentation and autosomal dominant inheritance of CPVT-CaM mutations and suggest that RyR2 interactions are unlikely to explain arrhythmogenicity of LQTS-CaM mutations.

Original languageEnglish (US)
Pages (from-to)1114-1124
Number of pages11
JournalCirculation Research
Volume114
Issue number7
DOIs
StatePublished - Mar 28 2014

Keywords

  • calcium
  • catecholaminergic polymorphic ventricular tachycardia
  • long QT syndrome
  • ryanodine receptor calcium release channel
  • sarcoplasmic reticulum

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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    Hwang, H. S., Nitu, F. R., Yang, Y., Walweel, K., Pereira, L., Johnson, C. N., Faggioni, M., Chazin, W. J., Laver, D., George, A. L., Cornea, R. L., Bers, D. M., & Knollmann, B. C. (2014). Divergent regulation of ryanodine receptor 2 calcium release channels by arrhythmogenic human calmodulin missense mutants. Circulation Research, 114(7), 1114-1124. https://doi.org/10.1161/CIRCRESAHA.114.303391