Divergent modes of clonal spread and intraperitoneal mixing in high-grade serous ovarian cancer

Andrew McPherson, Andrew Roth, Emma Laks, Tehmina Masud, Ali Bashashati, Allen W. Zhang, Gavin Ha, Justina Biele, Damian Yap, Adrian Wan, Leah M. Prentice, Jaswinder Khattra, Maia A. Smith, Cydney B. Nielsen, Sarah C. Mullaly, Steve Kalloger, Anthony Karnezis, Karey Shumansky, Celia Siu, Jamie RosnerHector Li Chan, Julie Ho, Nataliya Melnyk, Janine Senz, Winnie Yang, Richard Moore, Andrew J. Mungall, Marco A. Marra, Alexandre Bouchard-Côté, C. Blake Gilks, David G. Huntsman, Jessica N. McAlpine, Samuel Aparicio, Sohrab P. Shah

Research output: Contribution to journalArticlepeer-review

163 Scopus citations


We performed phylogenetic analysis of high-grade serous ovarian cancers (68 samples from seven patients), identifying constituent clones and quantifying their relative abundances at multiple intraperitoneal sites. Through whole-genome and single-nucleus sequencing, we identified evolutionary features including mutation loss, convergence of the structural genome and temporal activation of mutational processes that patterned clonal progression. We then determined the precise clonal mixtures comprising each tumor sample. The majority of sites were clonally pure or composed of clones from a single phylogenetic clade. However, each patient contained at least one site composed of polyphyletic clones. Five patients exhibited monoclonal and unidirectional seeding from the ovary to intraperitoneal sites, and two patients demonstrated polyclonal spread and reseeding. Our findings indicate that at least two distinct modes of intraperitoneal spread operate in clonal dissemination and highlight the distribution of migratory potential over clonal populations comprising high-grade serous ovarian cancers.

Original languageEnglish (US)
Pages (from-to)758-767
Number of pages10
JournalNature Genetics
Issue number7
StatePublished - Jul 1 2016
Externally publishedYes

ASJC Scopus subject areas

  • Genetics


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