Divergent astrovirus associated with neurologic disease in cattle

Linlin Li, Santiago S Diab, Sabrina McGraw, Bradd Barr, Ryan Traslavina, Robert Higgins, Tom Talbot, Patricia C Blanchard, Guillermo Rimoldi, Elizabeth Fahsbender, Brady Page, Tung Gia Phan, Chunlin Wang, Xutao Deng, Patricia Pesavento, Eric Delwart

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Using viral metagenomics of brain tissue from a young adult crossbreed steer with acute onset of neurologic disease, we sequenced the complete genome of a novel astrovirus (BoAstV-NeuroS1) that was phylogenetically related to an ovine astrovirus. In a retrospective analysis of 32 cases of bovine encephalitides of unknown etiology, 3 other infected animals were detected by using PCR and in situ hybridization for viral RNA. Viral RNA was restricted to the nervous system and detected in the cytoplasm of affected neurons within the spinal cord, brainstem, and cerebellum. Microscopically, the lesions were of widespread neuronal necrosis, microgliosis, and perivascular cuffing preferentially distributed in gray matter and most severe in the cerebellum and brainstem, with increasing intensity caudally down the spinal cord. These results suggest that infection with BoAstV-NeuroS1 is a potential cause of neurologic disease in cattle.

Original languageEnglish (US)
Pages (from-to)1385-1392
Number of pages8
JournalEmerging Infectious Diseases
Volume19
Issue number9
DOIs
StatePublished - Sep 2013

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Viral RNA
Nervous System Diseases
Cerebellum
Brain Stem
Spinal Cord
Metagenomics
Encephalitis
Nervous System
In Situ Hybridization
Young Adult
Sheep
Cytoplasm
Necrosis
Genome
Neurons
Polymerase Chain Reaction
Brain
Infection
Gray Matter

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Epidemiology

Cite this

Divergent astrovirus associated with neurologic disease in cattle. / Li, Linlin; Diab, Santiago S; McGraw, Sabrina; Barr, Bradd; Traslavina, Ryan; Higgins, Robert; Talbot, Tom; Blanchard, Patricia C; Rimoldi, Guillermo; Fahsbender, Elizabeth; Page, Brady; Phan, Tung Gia; Wang, Chunlin; Deng, Xutao; Pesavento, Patricia; Delwart, Eric.

In: Emerging Infectious Diseases, Vol. 19, No. 9, 09.2013, p. 1385-1392.

Research output: Contribution to journalArticle

Li, L, Diab, SS, McGraw, S, Barr, B, Traslavina, R, Higgins, R, Talbot, T, Blanchard, PC, Rimoldi, G, Fahsbender, E, Page, B, Phan, TG, Wang, C, Deng, X, Pesavento, P & Delwart, E 2013, 'Divergent astrovirus associated with neurologic disease in cattle', Emerging Infectious Diseases, vol. 19, no. 9, pp. 1385-1392. https://doi.org/10.3201/eid1909.130682
Li, Linlin ; Diab, Santiago S ; McGraw, Sabrina ; Barr, Bradd ; Traslavina, Ryan ; Higgins, Robert ; Talbot, Tom ; Blanchard, Patricia C ; Rimoldi, Guillermo ; Fahsbender, Elizabeth ; Page, Brady ; Phan, Tung Gia ; Wang, Chunlin ; Deng, Xutao ; Pesavento, Patricia ; Delwart, Eric. / Divergent astrovirus associated with neurologic disease in cattle. In: Emerging Infectious Diseases. 2013 ; Vol. 19, No. 9. pp. 1385-1392.
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