Divalent cations activate TRPV1 through promoting conformational change of the extracellular region

Fan Yang, Linlin Ma, Xu Cao, KeWei Wang, Jie Zheng

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Divalent cations Mg2+ and Ba2+ selectively and directly potentiate transient receptor potential vanilloid type 1 heat activation by lowering the activation threshold into the room temperature range. We found that Mg2+ potentiates channel activation only from the extracellular side; on the intracellular side, Mg2+ inhibits channel current. By dividing the extracellularly accessible region of the channel protein into small segments and perturbing the structure of each segment with sequence replacement mutations, we observed that the S1-S2 linker, the S3-S4 linker, and the pore turret are all required for Mg2+ potentiation. Sequence replacements at these regions substantially reduced or eliminated Mg2+-induced activation at room temperature while sparing capsaicin activation. Heat activation was affected by many, but not all, of these structural alternations. These observations indicate that extracellular linkers and the turret may interact with each other. Site-directed fluorescence resonance energy transfer measurements further revealed that, like heat, Mg2+ also induces structural changes in the pore turret. Interestingly, turret movement induced by Mg2+ precedes channel activation, suggesting that Mg2+-induced conformational change in the extracellular region most likely serves as the cause of channel activation instead of a coincidental or accommodating structural adjustment.

Original languageEnglish (US)
Pages (from-to)91-103
Number of pages13
JournalJournal of General Physiology
Volume143
Issue number1
DOIs
StatePublished - Jan 2014

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Divalent Cations
Hot Temperature
Fluorescence Resonance Energy Transfer
Temperature
Capsaicin
Mutation
Proteins

ASJC Scopus subject areas

  • Physiology

Cite this

Divalent cations activate TRPV1 through promoting conformational change of the extracellular region. / Yang, Fan; Ma, Linlin; Cao, Xu; Wang, KeWei; Zheng, Jie.

In: Journal of General Physiology, Vol. 143, No. 1, 01.2014, p. 91-103.

Research output: Contribution to journalArticle

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