Distribution of particulate matter and tissue remodeling in the human lung

Kent E Pinkerton, Francis H Y Green, Cathy Saiki, Val Vallyathan, Charles Plopper, Venu Gopal, Daniel Hung, Emily B. Bahne, Susan S. Lin, Margaret G. Ménache, Marc B Schenker

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

We examined the relationship betweeen intrapulmonary particle distribution of carbonaceous and mineral dusts and remodeling of the airways along anatomically distinct airway paths in the lungs of Hispanic males from the central valley of California. Lung autopsy specimens from the Fresno County Coroner's Office were prepared by intratracheal instillation of 2% glutaraldehyde at 30 cm H2O pressure. Two distinct airway paths into the apico-posterior and apico-anterior portions of the left upper lung lobe were followed. Tissue samples for histologic analysis were generally taken from the intrapulmonary second, fourth, sixth, and ninth airway generations. Parenchymal tissues beyond the 12th airway generation of each airway path were also analyzed. There was little evidence of visible particle accumulation in the larger conducting airways (generations 2-6), except in bronchial-associated lymphoid tissues and within peribronchial tissue. In contrast, terminal and respiratory bronchioles arising from each pathway revealed varying degrees of wall thickening and remodeling. Walls with marked thickening contained moderate to heavy amounts of carbonaceous and mineral dusts. Wall thickening was associted with increases in collagen and interstitial inflammatory cells, including dust-laden macrophages. These changes were significantly greater in first-generation respiratory bronchioles compared to second- and third- generation respiratory bronchioles. These findings suggests that accumulation of carbonaceous and mineral dust in the lungs is significantly affected by lung anatomy with the greatest retention in centers of lung acini. Furthermore, there is significant remodelling of this transitional zone in humans exposed to ambient particulate matter.

Original languageEnglish (US)
Pages (from-to)1063-1069
Number of pages7
JournalEnvironmental Health Perspectives
Volume108
Issue number11
StatePublished - 2000

Fingerprint

Particulate Matter
Bronchioles
particulate matter
Tissue
dust
Dust
Lung
Minerals
mineral
collagen
anatomy
Airway Remodeling
Coroners and Medical Examiners
Glutaral
Lymphoid Tissue
Macrophages
Hispanic Americans
valley
distribution
tissue

Keywords

  • Asthma
  • California
  • Fibrosis
  • Lung pathology
  • Particulate matter
  • Pigmentation
  • PM
  • PM

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health

Cite this

Distribution of particulate matter and tissue remodeling in the human lung. / Pinkerton, Kent E; Green, Francis H Y; Saiki, Cathy; Vallyathan, Val; Plopper, Charles; Gopal, Venu; Hung, Daniel; Bahne, Emily B.; Lin, Susan S.; Ménache, Margaret G.; Schenker, Marc B.

In: Environmental Health Perspectives, Vol. 108, No. 11, 2000, p. 1063-1069.

Research output: Contribution to journalArticle

Pinkerton, KE, Green, FHY, Saiki, C, Vallyathan, V, Plopper, C, Gopal, V, Hung, D, Bahne, EB, Lin, SS, Ménache, MG & Schenker, MB 2000, 'Distribution of particulate matter and tissue remodeling in the human lung', Environmental Health Perspectives, vol. 108, no. 11, pp. 1063-1069.
Pinkerton, Kent E ; Green, Francis H Y ; Saiki, Cathy ; Vallyathan, Val ; Plopper, Charles ; Gopal, Venu ; Hung, Daniel ; Bahne, Emily B. ; Lin, Susan S. ; Ménache, Margaret G. ; Schenker, Marc B. / Distribution of particulate matter and tissue remodeling in the human lung. In: Environmental Health Perspectives. 2000 ; Vol. 108, No. 11. pp. 1063-1069.
@article{9c92e67e7a934f87b1ef56cdcac7f73d,
title = "Distribution of particulate matter and tissue remodeling in the human lung",
abstract = "We examined the relationship betweeen intrapulmonary particle distribution of carbonaceous and mineral dusts and remodeling of the airways along anatomically distinct airway paths in the lungs of Hispanic males from the central valley of California. Lung autopsy specimens from the Fresno County Coroner's Office were prepared by intratracheal instillation of 2{\%} glutaraldehyde at 30 cm H2O pressure. Two distinct airway paths into the apico-posterior and apico-anterior portions of the left upper lung lobe were followed. Tissue samples for histologic analysis were generally taken from the intrapulmonary second, fourth, sixth, and ninth airway generations. Parenchymal tissues beyond the 12th airway generation of each airway path were also analyzed. There was little evidence of visible particle accumulation in the larger conducting airways (generations 2-6), except in bronchial-associated lymphoid tissues and within peribronchial tissue. In contrast, terminal and respiratory bronchioles arising from each pathway revealed varying degrees of wall thickening and remodeling. Walls with marked thickening contained moderate to heavy amounts of carbonaceous and mineral dusts. Wall thickening was associted with increases in collagen and interstitial inflammatory cells, including dust-laden macrophages. These changes were significantly greater in first-generation respiratory bronchioles compared to second- and third- generation respiratory bronchioles. These findings suggests that accumulation of carbonaceous and mineral dust in the lungs is significantly affected by lung anatomy with the greatest retention in centers of lung acini. Furthermore, there is significant remodelling of this transitional zone in humans exposed to ambient particulate matter.",
keywords = "Asthma, California, Fibrosis, Lung pathology, Particulate matter, Pigmentation, PM, PM",
author = "Pinkerton, {Kent E} and Green, {Francis H Y} and Cathy Saiki and Val Vallyathan and Charles Plopper and Venu Gopal and Daniel Hung and Bahne, {Emily B.} and Lin, {Susan S.} and M{\'e}nache, {Margaret G.} and Schenker, {Marc B}",
year = "2000",
language = "English (US)",
volume = "108",
pages = "1063--1069",
journal = "Environmental Health Perspectives",
issn = "0091-6765",
publisher = "Public Health Services, US Dept of Health and Human Services",
number = "11",

}

TY - JOUR

T1 - Distribution of particulate matter and tissue remodeling in the human lung

AU - Pinkerton, Kent E

AU - Green, Francis H Y

AU - Saiki, Cathy

AU - Vallyathan, Val

AU - Plopper, Charles

AU - Gopal, Venu

AU - Hung, Daniel

AU - Bahne, Emily B.

AU - Lin, Susan S.

AU - Ménache, Margaret G.

AU - Schenker, Marc B

PY - 2000

Y1 - 2000

N2 - We examined the relationship betweeen intrapulmonary particle distribution of carbonaceous and mineral dusts and remodeling of the airways along anatomically distinct airway paths in the lungs of Hispanic males from the central valley of California. Lung autopsy specimens from the Fresno County Coroner's Office were prepared by intratracheal instillation of 2% glutaraldehyde at 30 cm H2O pressure. Two distinct airway paths into the apico-posterior and apico-anterior portions of the left upper lung lobe were followed. Tissue samples for histologic analysis were generally taken from the intrapulmonary second, fourth, sixth, and ninth airway generations. Parenchymal tissues beyond the 12th airway generation of each airway path were also analyzed. There was little evidence of visible particle accumulation in the larger conducting airways (generations 2-6), except in bronchial-associated lymphoid tissues and within peribronchial tissue. In contrast, terminal and respiratory bronchioles arising from each pathway revealed varying degrees of wall thickening and remodeling. Walls with marked thickening contained moderate to heavy amounts of carbonaceous and mineral dusts. Wall thickening was associted with increases in collagen and interstitial inflammatory cells, including dust-laden macrophages. These changes were significantly greater in first-generation respiratory bronchioles compared to second- and third- generation respiratory bronchioles. These findings suggests that accumulation of carbonaceous and mineral dust in the lungs is significantly affected by lung anatomy with the greatest retention in centers of lung acini. Furthermore, there is significant remodelling of this transitional zone in humans exposed to ambient particulate matter.

AB - We examined the relationship betweeen intrapulmonary particle distribution of carbonaceous and mineral dusts and remodeling of the airways along anatomically distinct airway paths in the lungs of Hispanic males from the central valley of California. Lung autopsy specimens from the Fresno County Coroner's Office were prepared by intratracheal instillation of 2% glutaraldehyde at 30 cm H2O pressure. Two distinct airway paths into the apico-posterior and apico-anterior portions of the left upper lung lobe were followed. Tissue samples for histologic analysis were generally taken from the intrapulmonary second, fourth, sixth, and ninth airway generations. Parenchymal tissues beyond the 12th airway generation of each airway path were also analyzed. There was little evidence of visible particle accumulation in the larger conducting airways (generations 2-6), except in bronchial-associated lymphoid tissues and within peribronchial tissue. In contrast, terminal and respiratory bronchioles arising from each pathway revealed varying degrees of wall thickening and remodeling. Walls with marked thickening contained moderate to heavy amounts of carbonaceous and mineral dusts. Wall thickening was associted with increases in collagen and interstitial inflammatory cells, including dust-laden macrophages. These changes were significantly greater in first-generation respiratory bronchioles compared to second- and third- generation respiratory bronchioles. These findings suggests that accumulation of carbonaceous and mineral dust in the lungs is significantly affected by lung anatomy with the greatest retention in centers of lung acini. Furthermore, there is significant remodelling of this transitional zone in humans exposed to ambient particulate matter.

KW - Asthma

KW - California

KW - Fibrosis

KW - Lung pathology

KW - Particulate matter

KW - Pigmentation

KW - PM

KW - PM

UR - http://www.scopus.com/inward/record.url?scp=0033675743&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033675743&partnerID=8YFLogxK

M3 - Article

C2 - 11102298

AN - SCOPUS:0033675743

VL - 108

SP - 1063

EP - 1069

JO - Environmental Health Perspectives

JF - Environmental Health Perspectives

SN - 0091-6765

IS - 11

ER -